Abstract:Chitin is a promising structural material for biomedical applications, due to its many advantageous properties and abundance in nature. However, its usage and development in the biomedical field have been stagnant, because of chitin's poor mechanical properties in wet conditions and the difficulties in transforming it into an applicable form. To overcome these challenges, we created a novel biomimetic chitin composite. This regenerated chitin, prepared with ionic liquid, showed improved mechanical properties in wet conditions by mimicking insect cuticle and squid beak sclerotization, i.e., catechol-meditated cross-linking. By ionic liquid-based heat treatment, dopamine oxidation produced melanin-like compounds and dopamine-meditated cross-links without any solvent evaporation and oxidant utilization. The dopamine-meditated sclerotization increased the ultimate tensile strength (UTS) of the regenerated chitin by 2.52-fold, measured after six weeks of phosphate-buffered saline (PBS) submersion. In addition, the linear swelling ratio (LSR) of the chitin film was reduced by about 22%. This strategy raises a possibility of using regenerated chitin as an artificial hard tissue in wet conditions.
Low interfacial energy, an intrinsic property of complex
coacervate,
enables the complex coacervate to easily encapsulate desired cargo
substances, making it widely used in encapsulation applications. Despite
this advantage, the low interfacial energy of the complex coacervate
makes it unstable against mechanical mixing, and changes in pH and
salt concentration. Hence, a chemical cross-linker is usually added
to enhance the stability of the complex coacervate at the expense
of sacrificing all intrinsic properties of the coacervate, including
phase transition of the coacervate from liquid to solid. In this study,
we observed an abrupt increase in the interfacial energy of the coacervate
phase in mineral oil. By controlling the interfacial energy of the
coacervate phase using a microfluidic device, we successfully created
double engulfed PEG-diacrylate (PEGDA) coacervate microparticles,
named DEPOT, in which the coacervate is engulfed in a cross-linked
PEGDA shell. The engulfed coacervate remained as a liquid phase, retained
its original low interfacial energy property to encapsulate the desired
cargo substances, and infiltrated into the target site by a simple
solvent exchange from oil to water.
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