Highlights
Rapid SARS-CoV-2 detection without RNA extraction.
Universal direct RT-qPCR protocol suitable for all respiratory materials.
Significant correlation of Ct values between direct and RNA RT-qPCR.
High SARS-CoV-2 detection rate by direct RT-qPCR of 95.8 % for Ct values <35.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.