9Wheat bran oil (WBO) has been reported to have an important content of bioactive 10 compounds such as tocopherols, alkylresorcinols, steryl ferulates and other phenolic 11 compounds; however, its poor solubility in water systems restricts its applications in the food 12 industry. This study is focused on the formulation of oil-in-water (O/W) nanoemulsions of 13 WBO in order to improve the bioaccessibility of its active compounds. The influence of oil 14 concentration, surfactant type and concentration, and emulsification method, on the droplet 15 size and stability of the nanoemulsions was investigated. Response surface methodology was 16 used to optimize the conditions for preparing stable nanoemulsions with the minimum droplet 17 size. The optimal nanoemulsion was obtained when 1% of WBO and 7.3% of a surfactant 18 * Author to whom correspondence should be addressed.

The supercritical fluid extraction of corn germ oil has been studied in this work.Extractions were carried out at different pressure, temperature and flow rate to analyse the influence of these variables on the extraction kinetics and the oil quality obtained.Extraction curves are initially linear with a slope close to the oil solubility value in supercritical CO 2 . Based on these results a mathematical model was successfully applied to describe the extraction curves. Characterization of supercritical crude corn oil was performed by determining some physical parameters such as refraction index, density and color. Additionally, the fatty acid composition, neutral lipids, the content of tocopherols, acid index, peroxide value, antioxidant capacity and the oxidative stability * Corresponding author 3 were determined in the corn oil extracted. Fatty acid composition was compared with that for crude germ oil and no significant differences between the oils extracted by both methods were found. Oxidative stability test using the Rancimat showed that supercritical CO 2 extracted corn oil is less protected against oxidation than n-hexane extracted oils.

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9The supercritical fluid extraction (SFE) of wheat bran alkylresorcinols has been studied. 10Extractions were carried out at 40.0 MPa. The effect of particle size, static extraction 11 pretreatment with supercritical CO 2 (SC-CO 2 ) and extraction temperature on the 12 extraction kinetics was investigated. The extraction yield increased as the particle size 13 decreased and with temperature. Extraction curves present a faster and linear initial 14 extraction period followed by a slower extraction period. Based on these results the 15 approximate mathematical model of Sovová was successfully applied to describe the 16 extraction curves. The total content of alkylresorcinols was determined and compared 17 with the alkylresorcinol content obtained by conventional organic solvent extraction. 18Due to the amphiphilic nature of these resorcinolic lipids, the extraction yield was 19 * Corresponding author 3 higher for polar organic solvents than for SC-CO 2 . Characterization of supercritical 20 extracts was also performed by determining the fatty acid composition and antioxidant 21 activity. 22 Keywords 23Supercritical fluid extraction. Wheat Bran. Alkylresorcinols. Sovová's model 24

The supercritical fluid extraction (SFE) of wheat bran oil with pure supercritical carbon dioxide at different extraction pressure (25, 40 and 55 MPa) and temperature (40, 70 and 95 ºC) has been studied. Since wheat bran is characterized by having an important content of bioactive compounds such as alkylresorcinols (AR) and other phenolic compounds, the content of such compounds in the extracted oil was evaluated in order to establish oil quality. The influence of extraction pressure and temperature and wheat bran moisture on the extraction yield and oil quality was studied. Oil quality was evaluated through parameters such as AR content and profile, total polyphenols index (TPI), antioxidant activity and fatty acids profile. SFE performed over fresh wheat bran at 55 MPa and 95 ºC was found to provide wheat bran oil with the highest AR and phenolic content and antioxidant capacity of all the runs performed in this work. Some SFE experiments focused on oil fractionation showed that this technique can provide oil fractions enriched with AR and phenolic compounds.