Imaging depth of field is shallow in applications with high magnification and high numerical aperture, such as microscopy, resulting in images with in- and out-of-focus regions. Therefore, methods to extend depth of field are of particular interest. Researchers have previously shown the advantages of using freeform components to extend depth of field, with each optical system requiring a specially designed phase plate. In this paper we present a method to enable extended depth-of-field imaging for a range of numerical apertures using freeform phase plates to create variable cubic wavefronts. The concept is similar to an Alvarez lens which creates variable spherical wavefronts through the relative translation of two transmissive elements with XY polynomial surfaces. We discuss design and optimization methods to enable extended depth of field for lenses with different numerical aperture values by considering through-focus variation of the point spread function and compare on- and off-axis performance through multiple metrics.
Shallow depth of field in imaging systems with high numerical apertures results in images with in- and out-of-focus regions. Therefore, methods to enhance the depth of field are of special interest. In point spread function engineering, a custom phase plate is designed for each system to reduce sensitivity to defocus and thereby extend depth of field. In this paper, we present a method that enables extended depth of field for a range of numerical apertures using a freeform variable logarithmic phase plate pair. We leverage a numerical design approach for the variable phase plate pair design, and explore phase plate optimization and performance by quantifying and comparing through-focus point spread function variation, and on- and off-axis performance for the designed phase plates.
We discuss and analyze the sensitivity to misalignment errors of a freeform phase plate pair designed to enable variable extended depth of field imaging. Methods and impacts of the errors on system performance are demonstrated.
Point spread function engineering uses specially designed phase plates placed at the exit pupil of an imaging system to reduce defocusing sensitivity. A custom phase plate is typically required for each system to enable extended depth of field imaging, so methods enabling variable extended depth of field imaging are of particular interest. In this paper, we discuss the fabrication of previously designed fixed cubic phase plates and variable phase plate pairs with quartic surface profiles and present a novel application of a point source microscope for performance characterization. Experimental measurements of through-focus point spread functions are compared with predictions to demonstrate and characterize the extended depth of field for both fixed and variable freeform phase plates.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.