Buckwheat is a pseudo-cereal widely grown and consumed throughout the world. Buckwheat is recognized as a good source of nutrients and, in combination with other health-promoting components, is receiving increasing attention as a potential functional food. Despite the high nutritional value of buckwheat, a variety of anti-nutritional features makes it difficult to exploit its full potential. In this framework, sprouting (or germination) may represent a process capable of improving the macromolecular profile, including reducing anti-nutritional factors and/or synthesizing or releasing bioactives. This study addressed changes in the biomolecular profile and composition of buckwheat that was sprouted for 48 and 72 h. Sprouting increased the content of peptides and free-phenolic compounds and the antioxidant activity, caused a marked decline in the concentration of several anti-nutritional components, and affected the metabolomic profile with an overall improvement in the nutritional characteristics. These results further confirm sprouting as a process suitable for improving the compositional traits of cereals and pseudo-cereals, and are further steps towards the exploitation of sprouted buckwheat as a high-quality ingredient in innovative products of industrial interest.

Docosahexaenoic acid (DHA) is a polyunsaturated fatty acid that benefits the prevention of chronic diseases. Due to its high unsaturation, DHA is vulnerable to free radical oxidation, resulting in several unfavorable effects, including producing hazardous metabolites. However, in vitro and in vivo investigations suggest that the relationship between the chemical structure of DHA and its susceptibility to oxidation may not be as clear-cut as previously thought. Organisms have developed a balanced system of antioxidants to counteract the overproduction of oxidants, and the nuclear factor erythroid 2-related factor 2 (Nrf2) is the key transcription factor identified for transmitting the inducer signal to the antioxidant response element. Thus, DHA might preserve the cellular redox status promoting the transcriptional regulation of cellular antioxidants through Nrf2 activation. Here, we systematically summarize the research on the possible role of DHA in controlling cellular antioxidant enzymes. After the screening process, 43 records were selected and included in this review. Specifically, 29 studies related to the effects of DHA in cell cultures and 15 studies concerned the effects of consumption or treatment with DHA in animal. Despite DHA’s promising and encouraging effects at modulating the cellular antioxidant response in vitro/in vivo, some differences observed among the reviewed studies may be accounted for by the different experimental conditions adopted, including the time of supplementation/treatment, DHA concentration, and cell culture/tissue model. Moreover, this review offers potential molecular explanations for how DHA controls cellular antioxidant defenses, including involvement of transcription factors and the redox signaling pathway.

Agrobiodiversity conservation includes strategies and actions to be taken to prevent landrace loss, a worldwide problem. Landraces are local varieties that have agricultural, cultural, and historical value but most of these are not studied yet. This research aimed to study the nutritional and phytochemical characteristics of the “Copafam” bean. In addition, the sensory properties and consumers’ hedonic ratings in a model food formulation (biscuits) made by this landrace have been examined. The results show that “Copafam” had a high dietary fiber content (34.83 ± 2.48 g/100 g dw) and it resulted in a great source of secondary metabolites as polyphenols (121.36 ± 5.31 mg GAE/g dw), flavonoids (6.51 ± 0.17 mg/kg dw), and anthocyanins (28.11 ± 0.16 mg Cy3 G/kg dw), having remarkable antioxidant activity too. Biscuits made from “Copafam” bean flour were characterized by a darker color and crunchy texture, and it was considered acceptable by consumers. All these characteristics make it a resource of great interest for innovative forms of consumption like fortified foods. This research showed that landraces can represent a great resource for an innovative food industry aiming to preserve agrobiodiversity and promote the sustainable development of mountain areas.

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Review question / Objective: Evaluate the potential effect of DHA in regulating cellular antioxidant enzymes and hypothesizes possible molecular scenarios between DHA and Nrf2 in regulating cellular antioxidant defenses. Eligibility criteria: Chosen studies were published between 1998 and 2021 without restriction regarding pe-riod or publication status. Exclusion criteria were: (i) titles irrelevant to the research topic; (ii) abstract inappropriate or not related to the research topic; (iii) studies that used n-3 PUFAs rich oils which not allowed to discriminate the effect of DHA from other n-3 PUFAs; (iv) studies that co-administrated DHA with other compounds; (v) studies that used DHA oxidation products to better reflect normal nutritional conditions (vi) studies or data with inadequate statistical analysis or inappropriate control. Reviews, letters, ab-stracts, and articles without a complete text in the English language were also excluded.