BackgroundThe usefulness of oral fluid (OF) sampling for surveillance of infections in pig populations is already accepted but its value as a tool to support investigations of porcine respiratory disease complex (PRDC) has been less well studied. This study set out to describe detection patterns of porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), swine influenza virus type A (SIV) and Mycoplasma hyopneumoniae (M. hyo) among farms showing differing severity of PRDC.The study included six wean-to-finish pig batches from farms with historical occurrence of respiratory disease. OF samples were collected from six pens every two weeks from the 5th to the 21st week of age and tested by real time PCR for presence of PRRSV, SIV and M. hyo and by quantitative real time PCR for PCV2. Data was evaluated alongside clinical and post-mortem observations, mortality rate, slaughter pathology, histopathology, and immunohistochemistry testing data for PCV2 antigen where available.ResultsPRRSV and M. hyo were detectable in OF but with inconsistency between pens at the same sampling time and within pens over sequential sampling times. Detection of SIV in clinical and subclinical cases showed good consistency between pens at the same sampling time point with detection possible for periods of 2–4 weeks. Quantitative testing of OF for PCV2 indicated different patterns and levels of detection between farms unaffected or affected by porcine circovirus diseases (PCVD). There was good correlation of PCR results for multiple samples collected from the same pen but no associations were found between prevalence of positive test results and pen location in the building or sex of pigs.ConclusionsDetection patterns for PRRSV, SIV and M. hyo supported the effectiveness of OF testing as an additional tool for diagnostic investigation of PRDC but emphasised the importance of sampling from multiple pens and on multiple occasions. Preliminary evidence supported the measurement of PCV2 load in pooled OF as a tool for prediction of clinical or subclinical PCVD at farm level.

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SUMMARYDuring dormancy energy conservation is a key priority and as such dormant animals undergo a major metabolic depression to conserve their limited endogenous fuel supplies. Mitochondrial coupling efficiency, the efficiency with which mitochondria convert oxygen into ATP, significantly affects aerobic metabolism and thus to maximise energy savings during dormancy it has been hypothesised that coupling efficiency should increase. However, previous studies have shown coupling efficiency to be maintained or even to decrease. In this study we measured state 3 and state 4 mitochondrial respiration in the muscle of the burrowing frog, Cyclorana alboguttata and calculated the respiratory control ratio as a measure of coupling efficiency. After 7 months in aestivation, C. alboguttata significantly reduced oxygen consumption of isolated mitochondria by 83% and, unlike other dormant animals, the frogs appeared to decrease rates of proton leak to a greater extent than ATP synthesis, consistent with an increase in mitochondrial coupling efficiency. The significant energy savings observed at the mitochondrial level were reflected at higher levels of biological organisation, with tissue oxygen consumption depressed by as much as 81% and whole animal metabolic rate by 82%. Cyclorana alboguttata can survive in a dormant state for several years and we propose the hypothesis that energy efficiency is increased during aestivation.

SUMMARY Although green striped burrowing frogs (Cyclorana alboguttata)experience large reductions in the mass and absorptive surface area of the small intestine (SI) during aestivation, little is known about how this may affect the functional capacity of the SI. We examined changes in the function(l-proline uptake rate and capacity) and metabolism of the SI(in vitro oxygen consumption, Na+/K+-ATPase activity and abundance) of C. alboguttata following 6 months of aestivation. l-Proline uptake rate was significantly higher in aestivating frogs, but overall uptake capacity was lower than in active frogs. Total SI oxygen consumption rate (VO2) was also lower in aestivating frogs, despite no difference in mass-specific V̇O2. The proportion of intestinal V̇O2 associated with Na+/K+-ATPase activity and protein synthesis was equivalent between active and aestivating frogs, suggesting these processes were unaffected by aestivation. Indeed, the activity of Na+/K+-ATPase transporters in the SI of aestivating frogs was not different from that of active animals. Aestivating frogs maintained Na+/K+-ATPase activity, despite experiencing a reduction in the density of Na+/K+-ATPase transporters, by increasing the molecular activity of the remaining pumps to 2–3 times that of active frogs. These results show that functionality of the SI is maintained at the cellular level, potentially facilitating the reclamation of nutrients from the intestinal lumen while in aestivation. Despite this, the functional capacity of the SI in aestivating C. alboguttata is significantly reduced due to a reduction in tissue mass,helping frogs to conserve energy while in aestivation.

Summary 1.We apply a cost-benefit model to investigate whether bimodal turtles change their diving behaviour in response to changing energetic costs of aerial vs. aquatic respiration. This question is significant both in the context of the evolution of specialized respiratory structures in bimodal turtles, and the ecological role of respiratory partitioning. 2. Elseya albagula is a bimodally respiring turtle that can extract oxygen from water via specialized cloacal bursae and can vary its reliance on aquatic respiration. We examined the effect of water depth, velocity and temperature on the surfacing frequency of E. albagula through analysis of submergence durations (dive length). We applied a model of resource gain maximization to predict that as the cost of surfacing increases there will be longer dives made possible by the increased reliance on aquatic respiration. Diving behaviour of juvenile turtles was recorded in a large observation tank at varying water depths (50, 100 and 150 cm) and temperatures (20, 25 and 30 ° C). Diving behaviour of adult turtles was also monitored in a flume at water velocities of 5, 15 and 30 cm s -1 . 3. Dive length significantly increased with water depth, with dives at 150 cm twofold longer than dives at 50 cm. Dive length was also significantly influenced by temperature, with shorter dives at 30 ° C than at 20 ° C. In contrast, water velocity had no effect on dive length or on the proportion of a trial adult E. albagula spent using a velocity refuge. 4. Juvenile E. albagula possess the flexibility to adjust dive length in response to changes in water depth and temperature in a manner we would expect if energy use were being optimized. We advance the proposition that the driving force for the evolution of specialized aquatic gas exchange structures in E. albagula is the reduced cost of transport to and from the surface.