Polysaccharides are ubiquitous in nature; they serve fundamental roles in vivo and are used for a multitude of food, pharmaceutical, cosmetic biomaterials, and biomedical applications. Here, the structure−property function for low acetylated Gellan gum hydrogels induced by divalent ions was established by means of optical, rheological, and microscopic techniques. The hydrogels interacted with visible light as revealed by birefringence and multiple scattering, as a consequence of quaternary, supramolecular fibrillar structures. The molecular assembly and structure were elucidated by statistical analysis and polymer physics concepts applied to high-resolution AFM height images and further supported by FTIR. This revealed intramolecular coil-to-single helix transitions, followed by lateral aggregation of single helices into rigid, fibrillar quaternary structures, ultimately responsible for gelation of the system. Calcium and magnesium chloride were shown to lead to fibrils up to heights of 6.0 nm and persistence lengths of several micrometers. The change in molecular structure affected the macroscopic gel stiffness, with the plateau shear modulus reaching ∼10 5 Pa. These results shed light on the two-step gelation mechanism of linear polysaccharides, their conformational molecular changes at the single polymer level and ultimately the macroscale properties of the ensued gels.
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A method
is designed to quickly form protein hydrogels, based on
the self-assembly of highly concentrated lysozyme solutions in acidic
conditions. Their properties can be easily modulated by selecting
the curing temperature. Molecular insights on the gelation pathway,
derived by in situ FTIR spectroscopy, are related to calorimetric
and rheological results, providing a consistent picture on structure–property
correlations. In these self-crowded samples, the thermal unfolding
induces the rapid formation of amyloid aggregates, leading to temperature-dependent
quasi-stationary levels of antiparallel cross β-sheet links,
attributed to kinetically trapped oligomers. Upon subsequent cooling,
thermoreversible hydrogels develop by the formation of interoligomer
contacts. Through heating/cooling cycles, the starting solutions can
be largely recovered back, due to oligomer-to-monomer dissociation
and refolding. Overall, transparent protein hydrogels can be easily
formed in self-crowding conditions and their properties explained,
considering the formation of interconnected amyloid oligomers. This
type of biomaterial might be relevant in different fields, along with
analogous systems of a fibrillar nature more commonly considered.
The utility of Ionic liquids (ILs) as alternative solvents for stabilizing and preserving for a long time the native structure of DNA may be envisaged for biotechnological and biomedical applications...
Hydrated ionic liquids (ILs) have been identified as solvent media able to enhance the structural stability of deoxyribonucleic acid (DNA). In this work, we investigate the molecular interaction between imidazolium-based ILs and DNA during its thermal unfolding pathway, by exploiting synchrotron-based UV Resonance Raman scattering (UVRR) experiments. This technique gives a selective focus on the thermal responses of specific nucleobases in the structure of DNA providing the experimental sensitivity to both cooperative and local structural changes occurring along the complex unfolding process of DNA. UVRR measurements probe two distinct temperature-dependent phenomena occurring in the DNA double-helix, i.e. a non-cooperative pre-melting mainly involving adenine bases and a cooperative melting transition primarily localized on guanine tracts. The analysis of Raman spectra reveals that both the cation and anion of the ionic liquids strongly interact with the structure of DNA, thus affecting the melting process but not perturbing the pre-melting transition that precedes the complete separation of the strands of DNA. Overall these results suggest that the dominant interaction occurs between the imidazolium cation and the bases of guanine and thymine in the structure of DNA, in agreement with previous results of molecular dynamics simulations.
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