Rice is among the 3 most important crops worldwide. While much of the world's rice harvest is based on modern high-yield varieties, traditional varieties of rice grown by indigenous groups have great importance as a resource for future crop improvement. These local landraces represent an intermediate stage of domestication between a wild ancestor and modern varieties and they serve as reservoirs of genetic variation. Such genetic variation is influenced both by natural processes such as selection and drift, and by the agriculture practices of local farmers. How these processes interact to shape and change the population genetics of landrace rice is unknown. Here, we determine the population genetic structure of a single variety of landrace rice, Bue Chomee, cultivated by Karen people of Thailand. Microsatellite markers reveal high level of genetic variation despite predominant inbreeding in the crop. Bue Chomee rice shows slight but significant genetic differentiation among Karen villages. Moreover, genetically determined traits such as flowering time can vary significantly among villages. An unanticipated result was the overall pattern of genetic differentiation across villages which conforms to an isolation by distance model of differentiation. Isolation by distance is observed in natural plant species where the likelihood of gene flow is inversely related to distance. In Karen rice, gene flow is the result of farmers' seed sharing networks. Taken together, these data suggest that landrace rice is a dynamic genetic system that responds to evolutionary forces, both natural and those imposed by humans.Oryza sativa ͉ geographical differentiation ͉ farmers' management ͉ local adaption ͉ crop genetic diversity

Rice grain breakage during milling is a problem in many parts of Asia. It has been suggested that nitrogen (N) fertilizer can improve the milling quality of rice. Therefore, this study investigates effects of N fertilization on grain N concentration, endosperm storage protein distribution, and milling quality of rice. Four Thai extra long grain commercial rice cultivars (KDML105, KLG1, PTT1, and CNT1) were grown at Chiang Mai University in the wet season of 2001 with 0 or 120 kg of N/ha at flowering. Anatomical sections showed that there was more storage protein accumulated in the lateral regions of polished grain of high N concentration than in grain of low N concentration. Percent (%) unbroken rice was positively correlated with relative abundance of storage protein in the lateral region of the endosperm in all cultivars. Applying N increased head rice N concentration in all cultivars, whereas % unbroken rice was increased in KLG1 and CNT1. KDML105 cultivar, on the other hand, already had high % unbroken rice and more abundant storage protein in the lateral region with the grain of low N concentration. It is hypothesized that high density of storage protein in the lateral region of the endosperm provides resilience and lessens grain breakage during milling. The additional protein may increase hardness in rice grains and thus could make the rice more resistant to breakage during milling. Furthermore, N fertilization may enhance the nutritional quality of rice grain by increasing the glutelin content, which is rich in lysine.

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Boron (B) deficiency depresses wheat, barley and triticale yield through male sterility. On the basis of field responses to B fertilization, maize (Zea mays L.) is affected by B deficiency in five continents. In a series of sand culture trials with maize subject to B0 (nil added B) and B20 (20 μM added B) treatments, we described how B deficiency depressed maize grain yield while showing an imperceptible effect on vegetative dry weight. With manual application of pollen to the silk of each plant, B0 plants produced 0.4 grain ear −1 compared with 410 grains ear −1 in B20 plants. Symptoms of B deficiency was observed only in B0 plants, which exhibited symptoms of narrow white to transparent lengthwise streaks on leaves, multiple but small and abnormal ears with very short silk, small tassels with some branches emerging dead, and small, shrivelled anthers devoid of pollen. Tassels, silk and pollen of B0 plants contained only 3-4 mg B kg −1 DW compared with twice or more B in these reproductive tissues in B20 plants. A cross-fertilization experiment showed that, although the tassels and pollen were more affected, the silk was more sensitive to B deficiency. Pollen from B20 plants applied to B0 silk produced almost no grains, while pollen from B0 on B20 silk increased the number of grains to 37% of the 452 grains plant −1 produced from B20 pollen on B20 silk. Therefore, the silk of the first ear may be targeted for precise diagnosis of B status at maize reproduction, for timely correction by foliar B application, and even for Befficient genotype selection.

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