India is a rich storehouse of medicinal plants. All natural products can be termed bioactive molecules, as every diverse molecule possesses one kind or multiple kinds of biological oblique pharmacological activities. The beauty about Natural Products (Herbal Drugs) libraries is the uniqueness in their chemical structural diversity and innovativeness that in compasses varied biological actions. Herbal drugs played an important role in drug discovery and were the basis of most early medicines. The major role of this is also seen in ontological, antihypertensive, immunosuppression and metabolic diseases. Since ages passed over traditional systems of medicine have depended on natural products derived from plant sources. There is a tremendous historical legacy of folklore uses of the plants in medicine. They have been used for the treatment of diseases in almost all the ancient civilization. 80% world population for the health care throughout the world is used nearly only 4% medicinal plants, while only a small percentage of which have been chemically investigated. Today India is a formidable force in generic world pharmaceutical market in the last 20 years, Indian chemist have made great contribution in developing cutting edge technology of active pharmaceutical ingredients. Hence taking all these things into consideration it create sufficient interest to carry out phytochemical analysis of leaves of Catharanthus roseus A. from Benoda region, due to the important pharmaceutical and medicinal properties of Catharanthus roseus A.
Stems and roots of Salacia genus plants have been used as a specific remedy for early-stage diabetes, and one of the four sulphonium sulphates, salacinol is the compound responsible for the anti-diabetic activity. Salacia is prone to microbial contamination and insect infestation; hence, methods to estimate the microbial load in such plants will enhance its nutritional value. This paper highlights the novel use of Soleris® to quantify microbes of all types, namely bacteria, yeasts, molds, and coliforms in herbal extracts. The microbial analysis results obtained with Soleris® test vial have been compared with the conventional method, and the results indicate that Soleris® is equally efficient as the conventional method and in fact displays several advantages over the traditional method. The Soleris® method is a real time monitoring system that is highly sensitive, user-friendly, and environmentally friendly since it generates very little biomedical waste and saves a large amount of time. The data presented here demonstrate that for highly contaminated samples, results are available within 24 h. For yeasts and molds, the Soleris® method produces results in 48 h, thus offering considerable time savings compared to other commonly used methods.
Objectives: This study aimed to identify the contaminating bacteria in the extract of Garcinia cambogia, which is regularly used as a dietary supplement for addressing obesity in humans. Methods: The Garcinia cambogia extract was used and experiments were conducted to isolate the contaminating bacteria and antibiotic susceptibility was tested. The organism was identified using BIOLOG system. Such an extract was used in a placebo-controlled animal study when 6 eight adult male rats weighing between 200 and 220 g were randomly distributed into three groups (n = 3) and in test group 1, a single dose of 100 mg/kg bw of Garcinia cambogia extract was given while in the test group 2, 100 mg Garcinia cambogia extract + 116 mg Picrorhiza kurroa extract were administered through oral gavage. The normal control rats were given distilled water, and the treatment lasted for 30 days. Blood plasma and liver tissues were prepared for biochemical analysis and histology studies.Results: Nearly ~10 3 cfu/g of Bacillus atrophaeus was present in the Garcinia cambogia extract and we demonstrate >99% reduction in the microbial load with tetracycline. Such an extract at a dose of 100 mg/kg, showed weight loss in Wistar rats when administered orally for 1 month with no significant changes in liver histopathology. Picrorhiza kurroa, also known for its hepatoprotective properties, has been administered at a dose of 116 mg/kg along with Garcinia extract at 100 mg/kg orally and found to improve levels of hepatic enzymes as similar to control animals, although not statistically signifi-How to cite this paper: Bhosale,
The present work is aimed to develop and validate a reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of Scopoletin, Bacopaside-II, Bacopasaponin-C, Withanolide-A, and Withanoside-IV in a proprietary polyherbal formulation containing Bacopa monnieri (Brahmi), Convolvulus pluricaulis (Shankhapushpi), Withania somnifera (Ashwagandha), Nardostachys jatamansi (Jatamansi), Myristica fragrans (Jatiphal) and Valeriana wallichii (Tagar) extracts intended for the treatment of insomnia. The HPLC analysis was performed on a Inertsil ODS, 3V, 250 x 4.6 mm x 5µm, C18 column using 0.1% orthophosphoric acid buffer as the mobile phase (solvent A) and acetonitrile (solvent B) with the gradient: 0-5 min, 10-20% B; 5-10 min, 20-30% B; 10-25 min, 30% B; 25-30 min, 30-40% B; 30-40 min, 40% B; 40-45 min, 40-60% B; 45-48 min, 60% B; 48-50 min, 60-30% B; 50-52 min, 30-10% B and 52-55 min, 10% B at a flow rate of 0.8 ml/min. The detection wavelength was chosen at 227 nm for Withanoside-IV and Withanolide-A, for Scopoletin, Bacopaside-II and Bacopasaponin C, it was 205 nm. The HPLC method was validated as per ICH guidelines for linearity, LOD and LOQ. The calibration curve of all the five phytomarkers showed excellent linear correlation coefficients with values (r2=0.996) for Scopoletin, (r2=0.995) for Withanoside-IV, (r2=0.996) for Withanolide-A, (r2=0.996) for Bacopaside-II and (r2=0.999) for Bacopasaponin-C. Limits of detection (LOD) were 0.04, 0.43, 0.35, 0.39 and 0.18 μg/ml and limits of quantification (LOQ) were 0.12, 1.29, 1.06, 1.18 and 0.54 μg/ml for Scopoletin, Withanoside-IV, Withanolide-A, Bacopaside-II and Bacopasaponin-C respectively. The developed HPLC method showed good separation of all the five constituents, enabling efficient analysis of Scopoletin, Withanoside-IV, Withanolide-A, Bacopaside-II, and Bacopasaponin-C in the polyherbal formulation
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.