<p class="MsoNormal"><span style="color: black; mso-themecolor: text1;">Wheatgrass</span> is a plethora of essential phytochemicals considered to exhibit numerous benefits on human health. Therefore, the study of phytochemicals with varying stages of growth is essential. This study aims to determine the optimum harvesting period of wheatgrass based on its phytochemical content. During the growth of wheat seed (WK 1204 variety), the phytochemicals such as chlorophyll, total phenol content, flavonoids and tannins were extracted by 80% acetone and 80% methanol from wheatgrass harvested on days 6, 7, 8, 9 12 and 15, respectively. <span style="background: white;">2,2-diphenyl-1-picrylhydrazyl</span> (DPPH) radical scavenging activity, chlorophyll and flavonoid contents significantly (p<0.05) increased and reached the maximum level on day 9. Total phenol content was found to be increasing significantly (p<0.05), while the tannin content was decreasing considerably (p<0.05) on day 15. The optimum harvesting period was found <span style="mso-ansi-language: IN;" lang="IN">on</span> day 9. The DPPH radical scavenging activity, chlorophyll and flavonoid content significantly (p<0.05) increas<span style="mso-ansi-language: IN;" lang="IN">ed</span> and reached the maximum level, i.e., 92.27±1.92%, 6.63±0.053 mg g<sup><span style="mso-ansi-language: IN;" lang="IN">-1</span></sup> and 183.64±33.49 mg QE g<sup><span style="mso-ansi-language: IN;" lang="IN">-1</span></sup>, respectively, on day 9 of cultivation. The total phenol content was found to be increasing significantly (p<0.05) from 291.67±5.69 mg GAE g<sup><span style="mso-ansi-language: IN;" lang="IN">-1 </span></sup>on day 6 to 446.67±5.77 mg GAE g<sup><span style="mso-ansi-language: IN;" lang="IN">-1</span></sup> on day 15, while the tannin content was declining significantly (p<0.05) from 11.74±0.29 mg GAE g<sup><span style="mso-ansi-language: IN;" lang="IN">-1</span></sup> on days 6, 7 and so on to 3.36±0.47 mg GAE g<sup><span style="mso-ansi-language: IN;" lang="IN">-1</span></sup> on day 15. Therefore, the optimum harvesting period of wheatgrass was found to be day 9 in terms of phytochemical analysis.</p>
Wheatgrass is an inexpensive and efficient source to provide all the required nutrients and medicinal benefits for a healthy body. The major objective of this study is to formulate a health-beneficial beverage that could be organoleptically accepted by people of all age groups, to bring forward a new product from wheatgrass that has mostly remained unexplored. The wheatgrass harvested on the 9th day (optimized on the basis of chlorophyll, tannin, total polyphenols, flavonoids and DPPH radical scavenging activity) was taken for study. The wheat seeds (WK 1204 variety), collected from Agriculture Botany Division, Nepal Agriculture Research Council were used for the preparation of ready-to-serve juice. Eight different formulations of RTS were prepared with the help of Design-Expert®, version 7, keeping juice content 7-13%, TSS 9-16 °Bx and constant acidity at 0.3%. Response Surface Methodology, D-optimal design was adapted for the formulations. The optimization of the formulation was intended to be carried out based on sensory analysis. Proximate and phytochemical (Chlorophyll, Polyphenol, Flavonoids, Tannins and Antioxidant properties) composition of 9th days harvested wheatgrass, its extracted juice and the final product was also carried out. The statistical analysis (two-way ANOVA, no blocking) was done in order to evaluate the outcomes of sensory analysis. There was a significant difference for sensory attributes like flavor, taste, body and overall acceptability at p<0.05, whereas color wasn’t found to be significantly different. Sensory analysis revealed that the beverage consisting of 12.8% Wheatgrass juice, 12.5°Bx TSS and 0.3% acidity was considered best among all the combinations.
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