The functionalization of liposomes with antibodies is a potential strategy to increase the specificity of liposomes and reduce side-effects of chemotherapeutic agents. The active targeting of Human Epidermal growth factor Receptor 2 (HER2) positive breast cancer cells can be achieved by coating liposomes with an anti-HER2 monoclonal antibody. In this study, we synthesized Calcein and Doxorubicin loaded immunoliposomes functionalized with the monoclonal antibody Trastuzumab. Both liposomes were characterized for size, phospholipid concentration and antibody conjugation. The effect of low-frequency ultrasound (LFUS)-induced drug release was tested using three power densities, 7.46, 9.85 and 17.31 mW/cm2; and the release data were modeled using six different kinetic models. LFUS results established the sonosensitivity of both carrier types, with immunoliposomes being more acoustically sensitive than control liposomes. Results also showed an increase in the release rate as the power density increased from 7.46 to 17.31 mW/cm2. Finally, the in vitro cell experiments showed enhanced uptake and cytotoxicity when breast cancer cell lines, SKBr3 and MDAMB-231, were treated with LFUS-triggered HER-liposomes.
Nanotechnology is a rapidly growing industry where nanomaterials are used in almost every field, including electronics, cosmetics, engineering, household products, biotechnology and medicine. Nanoparticles (NPs) have unique physical and chemical properties, which may cause potential hazards to human health, especially with constant exposure. Various studies have shown that NPs can enter the human body either through the respiratory tract, dermal absorption or via the gastrointestinal system and have the potential to cause respiratory disorders, behavioral changes, neurological disorders, as well as cancer. This review focuses on the health implications of NPs, specifically gold, silver, silica, titanium dioxide, aluminum, aluminum oxides, metal organic frameworks (MOF), aerosol particles, flame retardants, quantum dots, and carbon nanotubes. Herein, we discuss the routes of exposure and the impact of these nanoparticles on human health. We also summarize in-vitro and in-vivo studies that analyze the cytotoxicity profile and the associated health impact of these nanoparticles. This study could be utilized to develop well-defined guidelines for setting exposure limits for different NP types as well as a summary of related characteristics such as size, shape, morphology, and surface charge.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.