Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach.The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH 4 ) 2 SO 4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH 4 ) 2 SO 4 ) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.
Background: This study was conducted at a rural Community of Lahore Pakistan in 2021, to assess the different factors that affect place of delivery and delivery practices. The different factors that affect delivery choices were financial problems, gender role issues and traditional practices, cultural and ethnic values, access to health centers due to distance. Study design and method: A descriptive cross sectional quantitative study design was used. Data was collected from married females through convenient non probability sampling technique and interviews. 70 married females participated in this study. Results: Fear of hospital due to no exposure in health centers , lack of maternal education and cultural values major influence their delivery choice, According to the results approximately 70% of the population had cost issues and 58% preferred to deliver at home because of psychological satisfaction and convenience. Conclusion: The findings of the Research emphasize the need of building maternal health centers to overcome the cost and distance issues. Educating females regarding maternal health. Maternal health centers may effectively reduce the risk of precipitate delivery and traditional delivery practices.
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