KEYWORDS Mithun CryptorchidComputer assisted sperm analysis Motility Velocity parameters ABSTRACTThe present study was conducted to assess the motility and velocity parameters of sperm of normal intact and unilateral cryptorchid mithun bull by computer assisted sperm analyzer (CASA). Twenty ejaculates were collected from cryptorchid (n=10) and normal intact (n=10) bull. The different parameters assessed through CASA were static sperms (SM), Total Motility (TM), forward progressive motility (FPM), Average Path velocity (VAP), Average beat/cross frequency (BCF), Straightness (STR), Average straight line velocity (VSL), Average curvilinear velocity (VCL), Average amplitude of lateral head displacement (ALH), Wobble (WOB), Linearity (LIN), Elongation (EL). The result showed that these CASA parameters differed significantly (p<0.05) between the intact and cryptorchid mithun bull and intact mithun bull has significantly (p<0.05) higher value than cryptorchid mithun bulls. It was concluded that most of the CASA parameters were significantly lower in cryptorchid bulls than intact bull and however, due to the genetic hereditary nature of cryptorchid, these bulls should not be used for breeding purposes.
KEYWORDS Melatonin MithunComputer assisted sperm analysis Liquid semen preservation ABSTRACTPresent study was conducted to evaluate the effect of melatonin (MEL) on velocity and motility of mithun semen by computer assisted sperm analyser. Semen ejaculates (n=25) were collected from matured mithun bulls (n=10) using trans-rectal massage method and were divided and grouped into six aliquots equally, extended with the standard Tris Egg Yolk Citrate semen diluent. Six groups for various treatments were prepare, these group were control -semen without MEL (Gr 1), 1mM (Gr 2), 2mM (Gr 3), 3Mm (Gr 4), 4mM (Gr 5:) and 5mM (Gr 6). Various parameter such as total motility, forward progressive motility, straight line velocity, curvilinear velocity, average path velocity, wobble, linearity, straightness, beat/cross frequency, amplitude of lateral head displacement and velocity of rapid, medium, slow and static were measured for 0-30h at 6 hrs interval at 5 o C. The result observed that these mobility and velocity parameters were varied significantly (p<0.05) among the experimental periods and among the experimental groups. Further, MEL at 3 mM has significant (p<0.05) improvement in the mobility and velocity parameters than MEL at 1, 2, 4or 5 mM stored in in-vitro for up to 30 h of incubation. It was concluded that MEL 3 mM treated sperm has increased functional sperm structures faster to move and forward direction, probably improves the fertilization rate.
This short communication of a case report of dystocia in a pluriparous Nagaland mithun strain was due to fetal maldisposition and malpresentation. Severe left lateral deviation of head and neck and dorsopubic-anterior presentation of the fetus caused the maldisposition in Nagaland mithun strain. It was successfully removed with caesarean section and the animal recovered uneventfully.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.