The aim of this study was to evaluate the effect of reinforcing polyaromatic polyamide (aramid) fibers with various orientations on the flexural properties of denture base resin. Aramid fibers with four orientations of unidirectional, woven, non-woven and paper-type were pre-impregnated and placed at the bottom of a specimen mold. Heat-polymerized denture base resin was packed over the fibers and polymerized. A three-point bending test was performed using a universal testing machine at a crosshead speed of 5 mm/min. The flexural strengths and flexural moduli of the unidirectional and woven groups were significantly higher than those of the control and other experimental groups. For the flexural moduli, all experimental groups showed significantly higher reinforcing effects than the control group. In conclusion, the unidirectional group located perpendicular to the direction of the load was most effective in reinforcing the denture base resin, followed by the woven group.
We cultured human mesenchymal stem cells (hMSCs) on TiO2nanotubes with diameters of 30–100 nm to assess the size-effect of TiO2nanotubes on the behavior and osteogenic functionality of hMSCs. Most studies of the expression of genes encoding alkaline phosphatase (ALP), osteocalcin (OCN), osteopontin (OPN), and integrin-β (INT-B), after 1 week of incubation, supported the results of cell viability and MTT assays at 48 hrs of plating. However, after 2 weeks of incubation, expression of ALP, OPN, INT-B, and protein kinase R-like ER kinase (PERK) genes were significantly higher in cells cultured on 70 nm TiO2nanotubes than that in cells cultured on other TiO2nanotubes and Ti. This biphasic osteogenic characteristic of hMSCs is supposed to relating to the nature of the hMSCs adhering to the substrate at the beginning of incubation, and the nanostructural stimulation caused by the topography of TiO2nanotubes at a later stage of incubation. The discovery of these biphasic characteristics of hMSCs cultured on different-sized TiO2nanotubes may contribute to resolving the discrepant results relating to the size-effect of TiO2nanotubes on the adhesion, proliferation, and functionality of cells.
This study evaluated the shear bond strengths of various types of resin cements between three types of adherends (composite resin, metal, and ceramic) and bovine teeth with and without thermocycling. A conventional resin cement (Variolink N), two adhesive resin cements (PANAVIA F 2.0, Multilink N), and three self-adhesive resin cements (MAXCEM ELITE, Rely X Unicem 2, Speed CEM) were used. The adherends were cemented on the superficial dentin of bovine incisors using each resin cement. Herein, 10 specimens from each group were thermocycled 5,000 times, and the other 10 were stored without thermocycling. With the resin and ceramic adherends, the shear bond strengths of Rely X Unicem 2 were significantly higher than those of the other resin cements both with and without thermocycling (p<0.05). With the metal adherend, the shear bond strengths were not significantly different among the cement groups, except MAXCEM ELITE, which showed the lowest strength.
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