The aim of the study is to standardize the simple method for extracting DNA from cells fixed in fixative (3:1 ratio of methanol and acetic acid glacial) mostly used for chromosomal studies in cattle and buffaloes. These fixed cells were stored for more than 6 months at refrigerated temperature. The fixed cells were washed 2-3 times by the ice cold 1x Phosphate Buffer Saline (PBS) with pH 7.4, so that effect of fixative may be eliminated. The genomic DNA was extracted by adding cell lysis and nucleus lysis buffers. The quality and quantity of DNA were estimated. The readings of nano drop and agarose gel electrophoresis indicate good quality DNA isolated with a rapid and simple protocol routinely using in our laboratory. The method enables us to study the DNA of a cattle and buffaloes after completing cytogenetic investigation or in cases where DNA samples are otherwise not available. This protocol may be useful for molecular analysis of DNA from fixed cells palettes.
To investigate the chemeric condition of bulls by cytogenetic analysis the present study was conducted. The whole blood culture, cell harvesting, conventional Giemsa staining and GTG banding were performed for the chromosomal studies. During cytogenetic investigation on 200 buffalo bulls, 3 young bulls exhibited sex chromosomal chimerism (XX/XY) in their blood cells. Hundred metaphase fields were screened for each bull. Cytogenetic studies on chimeric bulls indicated unusual higher ratio of XX chromosomes in 3 bulls. The fertility status of these bulls are not known as they are young and not in semen collection. As documented, the fertility of chimeric bulls is not grossly affected and the condition is not inheritable. Hence, the bulls may be used for breeding programmes if their semen quality is good. DOI: http://dx.doi.org/10.3329/bjas.v42i1.15761 Bang. J. Anim. Sci. 2013. 42 (1): 20-22
Chromosomal aberrations are of great concern in dairy animals worldwide as they are usually associated with reduced fertility or infertility. There are multiple reasons which may cause chromosomal aberrations during mitosis and meiosis. To understand the probable cause of aberrations, a simple study was performed during induced lymphocyte culture. The lymphocyte cultures were setup using the blood samples of cattle and buffaloes. Samples were harvested without adding ethedium bromide and colchicine. The Giemsa stained chromosome slides were screened under the microscope (100X) and photographed with attached system.
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