A novel time-temperature indicator (ITI) using phospholipid-phospholipase was developed to monitor quality change of frozen foods during storage. The 'IT1 was more reliable than the lipase system and monitored quality changes of frozen pork during storage. The TTI was designed for reactions at sub-zero temperatures because the substrate emulsion would be stable at such temperatures. The lT1 contained glycerol and sorbitol as anti-freeze reagents and was designed to show distinctive color changes according to pH by mixing bromothymol blue, neutral red, and methyl red. The 'IT1 had an activation energy of 32.1.2 kcal/mol, suitable for many food reactions.
The interesterification reaction by immobilized lipase between triolein and behenic acid (BA) or ethyl behenate (EB) was investigated in supercritical carbon dioxide (SCCO2) to produce 1,3-dibehenoyl-2-oleoyl glycerol (BOB). The incorporation rate of behenoyl group to triolein was much higher with EB as a substrate than with BA. The solubility in SCCO2 was found to be significantly higher for EB than BA, which seemed to cause the higher formation rate of the behenoyl-enzyme complex in the former case to give the higher production rate of the final interesterification product, BOB.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.