O objetivo deste trabalho foi avaliar a relação entre a presença de folhas e o enraizamento de estacas de Alternanthera brasiliana. A. brasiliana é uma espécie herbácea nativa amplamente utilizada na medicina popular devido às suas propriedades analgésicas, cicatrizantes, anti-inflamatórias e antimicrobianas. A estaquia é uma técnica de reprodução vegetativa que oferece maior rapidez e eficiência na propagação de espécies vegetais. As estacas de A. brasiliana foram coletadas a partir de ramos herbáceos em março (outono) de 2019. As estacas foram preparadas com 15 cm de comprimento e divididas nos seguintes grupos experimentais: estacas sem folhas; e estacas com um e dois pares de folhas cortadas ao meio. Foram distribuídas 40 estacas, em quatro repetições, para cada grupo experimental. As estacas então foram plantadas em tubetes contendo uma mistura de substrato orgânico comercial e vermiculita (1:1), permanecendo em casa-de-sombra com irrigação semiautomatizada ao amanhecer e ao entardecer. As avaliações foram realizadas ao final de 47 dias, observando-se as seguintes variáveis: porcentagem de estacas enraizadas; estacas com calos; estacas vivas e mortas; presença e número de brotações; e comprimento e número médio de raízes. Foi possível concluir que ambos os parâmetros (presença e número de folhas) não influenciam no enraizamento de estacas de A. Brasiliana, o que é explicado pela alta produção endógena de hormônios de enraizamento, como a auxina. Dessa forma, conforme a literatura publicada, foi demonstrado que A. brasiliana pode ser considerada uma espécie de fácil enraizamento.
The rapid spread of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has led the world to a pandemic. Therefore, rapid, sensitive, and reproducible diagnostic tests are essential to indicate which measures should be taken during pandemics. We retrospectively tested unextracted nasopharyngeal samples from consecutive patients with suspected SARS-CoV-2 infection (n = 334), and compared two different Ct cut-off values for interpretation of results using a modified Allplex protocol. Its performance was evaluated using the USA Centers for Disease Control and Prevention (CDC) as reference. The reduction on Ct cut-off to 35 increased the test NPA from 79.65 to 88.00 %, reducing the number of false positives, from 10.48 to 6.29 %, resulting in an almost perfect agreement between the Allplex and the CDC protocol (Cohen’s Kappa coefficient = 0.830 ± 0.032). This study demonstrates that the Seegene Allplex™ 2019-nCoV protocol skipping the viral RNA extraction step using the Ct cut-off of 35 is a rapid and efficient method to detect SARS-CoV-2 in nasopharyngeal samples.
Several vaccines have been produced in 2 years of the COVID‐19 pandemic to control the infection outbreak. This study demonstrated the success of vaccination in controlling COVID‐19 cases and deaths in a small city (41 424 people) with a low population density in Brazil. This study was based on a 1‐year dataset since the application of the first dose in January 2021. The results show a reduction in positive cases and deaths as the vaccination coverage increased in the city, mainly after 15 000 inhabitants were vaccinated (35.21% of the population) in July 2021. At the time, 49.06% of administered vaccines were ChAdOx1‐S recombinant, 39.80% inactivated SARS‐CoV‐2 virus (CZ02 strain), 9.70% Tozinameran, and 1.44% Ad26.COV2‐S recombinant. From August 2021, a marked reduction in daily positive cases and deaths was observed, and incidence (≤2.49 per 1000 inhabitants) and mortality (≤0.02 per 1000 inhabitants) rates remained stabilized until January 2022, when a new outbreak occurred upon the emergence of the Omicron variant. However, the mortality rate (0.07 per 1000 inhabitants) remained low regardless of the Omicron high incidence rate (68.41 per 1000 inhabitants). These data demonstrate the COVID‐19 vaccination effectiveness with a threshold of 35.21% of the population vaccinated in this city model.
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