The coconut water is the most nutritious wholesome beverage in all coconut producing countries. Folk medicine describes the use of coconut water to reduce toxic effects of nicotine and alcohol in reproductive function in men, but its direct evidence is lacking. The aim of this study was to investigate the effects of tender and mature coconut water consumption on nicotine induced changes in sperm quality, spermatogenic cell density, activity of testicular marker enzymes for spermatogenesis and testosterone level of male healthy rats. Male Sprague Dawley rats were treated with tender and mature coconut water intragastrically and nicotine subcutaneously for 15 days. The findings indicated that the coconut water supplementation improves epididymal spermatogenic cell density (p ≤ 0.05), sperm motility and morphology which were altered by nicotine. The activity of acid phosphatase and alkaline phosphatase was increased (p ≤ 0.05), which indicates that treatment with coconut water provides significant protection of germinal epithelium from nicotine toxicity. Coconut water also shows a significant increase (p ≤ 0.05) in testosterone levels in nicotine treated rats. Coconut water ameliorates the reproductive toxicity caused by nicotine due to presence on nutrients L-arginine, ascorbic acid, minerals like calcium and magnesium.