Plants are fantastic sources for present day life saving drugs. Monocrotaline a natural ligand exhibits dose-dependent cytotoxicity with potent antineoplastic activity. This study was intended to disclose the therapeutic potential of monocrotaline against hepatocellular carcinoma. The in silico predictions have highlighted the antineoplastic potential, druglikeness and biodegradability of monocrotaline. The in silico docking study has provided an insight and evidence for the antineoplastic activity of monocrotaline against p53, HGF and TREM1 proteins which play a threatening role in causing hepatocellular carcinoma. The mode of action of monocrotaline was determined experimentally by in vitro techniques such as XTT assay, NRU assay and whole cell brine shrimp assay have further supported our in silico studies. The in vitro cytotoxicity of monocrotaline was proved at IC50 24.966 µg/mL and genotoxicity at 2 X IC50 against HepG2 cells. Further, the credible druglike properties with non-mutagenicity, non-toxic on mammalian fibroblast and the potential antineoplastic activity through in vitro experimental validations established monocrotaline as a novel scaffold for liver cancer with superior efficacy and lesser side effects.

Nightmares also categorized as fighting dreams suddenly awake the sleeper from Rapid Eye Movement (REM) sleep. Nightmares can be caused by improper food intake, alcohol withdrawal, digestive or nervous disorders and drug induced (mainly due to the drugs which affect the neurotransmitters). Some of the drugs that can induce nightmares are Beta blockers, Statins, Anti depressants, Anti epileptics, Sedative hypnotics, General anesthetic agents, anti histamines, endocrinal agents etc., The exact mechanisms involved for these drug induced nightmares is not clear. The occurrence of these nightmares due to the intake of various drugs is rare and in all these cases drug withdrawal is beneficial.

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In silico docking studies on 3-D structure generated using I-tasser with quinine, chloroquine, artesunate into the active site of apicoplast import protein Tic20 established apicoplast import protein Tic20 as a promising therapeutic molecular target.