Hyperthyroidism can be defined as the status of over production of thyroid hormones: tri-iodothyronine (T3) and thyroxine (T4) as a results of thyroid gland dysfunction. The aim of the current study was to investigate the association between the abnormalities of thyroid hormones (T3 and T4) and serum levels of thyroid auto-antibodies: anti-thyroid peroxidase (TPO-Ab) and anti-thyroid stimulating hormone receptor (TR-Ab) and the state of hyperthyroidism. Blood samples were collected from 75 Iraqi individualswere early diagnosed as hyperthyroidism patients in addition to 25 Iraqi individuals as a healthy control group. Enzyme Linked Fluorescent Assay (ELFA) used to determine the levels of thyroid hormones (T3 and T4) and the level of thyroid stimulating hormone (TSH), Enzyme Linked Immuno-Sorbent Assay (ELISA) was used to determine the levels of auto-antibodies (TPO-Ab and TR-Ab). The results show that T3 levels and T4 levels in hyperthyroidism patients (2.07 nmol/L and 14.02 μg/dl respectively) increased significantly (푃<0.01)compared to healthy control levels (1.33 nmol/L and 6.4 μg/dl respectively). While the levels of thyroid stimulating hormone (TSH) in hyperthyroidism patient (0.17 μIU/Ml) decreased significantly (푃<0.01)when compared with healthycontrol group (1.84 μIU/Ml). The results also showed that levels of TPO-Ab and TR-Ab in hyperthyroidism patients (235.29 IU/mL and 32.05 IU/mL respectively) increased significantly (푃<0.01)when compared to healthy control group (20.76 IU/mL and 0.3 IU/mL respectively). This study revealed that there is an association between thyroid hormones disorders and the positivity of thyroid auto-antibodies in hyperthyroidism patient
This study aim is to purify β-galactosidase from a local isolate of yogurt in Salah al-Din Governorate to overcome the phenomenon of lactose in decomposition. The bacteria were grown on MRS medium supplemented with 1%CaCo3. Twenty isolates of lactic acid bacteria were obtained and conducting culture tests and microscopic examinations were on these isolates. In order to classify them to the level of species, it was found that there were four types, namely: Lactobacilluse acidophilus,LactobacilluseCasei,Lactobacilluse delubrici subsp.bulgaricus,Streptococcus thermophilus, The cultivation and activation steps of the different isolates were carried out forobtaining the most productive and active isolate, which is Lactobacilluse acidophiluse. Beta-galactosidase activation processes were carried out for the enzyme and cell breakdown by lysozyme. Purification and sedimentation processes were carried out using ammonium sulfate and membrane sorting, followed by gel filtration using Lactobacillus G-150. The best extraction rate (L.acidophiluse 70%) was achieved by enzyme precipitation (4,375) units/mol, and the activity increased in the membrane sorting step to (5,900) units/mol, and in gel filtration we obtained activity of the enzyme (15.591) units/mol.
Type 1 diabetes mellitus (T1DM) is an autoimmune disease frequently associated with autoimmune thyroid disease (AITD). The study is conducted at the Specialized Center for Endocrinology and Diabetes-Baghdad at Al-karkh side, during December 2013 up to April 2014. In this study, we investigate the prevalence of anti-thyroid peroxidase (anti-TPO) antibody in(80) type1 diabetic patients with (AITD) and (30) healthy controls .Blood samples are taken for investigation of thyroid tests by using Vitek Immunodiagnstic Assay System (VIDAS).Enzeme Linked Immunosorbent Assay (ELISA) is used to detect anti-thyroid antibody(anti-TPO). The results show that age, gender and BMI (body mass index) are similar in both groups, p>0.05. Among 80 type1 diabetic patients 15(18.8%) are positive. There is a highly significant difference (p0.05) between T3 &T4 and frequency of positive and negative (anti-TPO).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.