No abstract

The present study aimed to discover the histopathological of the Monosodium glutamate (MSG), and Sodium nitrite (NaNO2), on the embryonic development of the eyes of albino mice Mus musculus. On the fourteenth and eighteenth day of pregnancy, the stage of organogenesis in these animals. A concentration of 9 g/kg of MSG, a concentration of 110 mg/kg of NaNO2, and the interaction between them used. The results of the study showed the presence of pathological changes to the eyes of the fetuses. The eye on the 14th day of pregnancy, when 9 g/kg of MSG used, there were retinal duplication, increased vascularization in the retina, condensation of some nuclei of the inner nuclear layer and ganglion cells, and necrosis in the vicinity of the lens. When treating with NaNO2 110 mg/kg, there was an irregularity in the lens, corneal distortion, hyperplasia of the retinal nerve tissue. When the two materials overlapped, the corneal tissue necrosis, the lens fiber, and the inner plexiform layer were observed. On the 18th day of pregnancy, when treated with MSG 9g/kg, the most significant overall and striking damage was retinal duplication and optic nerve necrosis. When treated with NaNO2 110 mg/kg, the corneal stroma and dissociation were seen in the photoreceptor cells. In the case of their overlapping, extensive necrosis and reduction appeared in all layers of the retina. The study concluded that consuming MSG and NaNO 2 more than the permissible limit during pregnancy leads to tissue lesions harmful to the eye.

Many studies are still the subject of food additives to know their positive and negative effects, primarily as they are widely used globally. Therefore, this study aimed to identify the histological effects of sodium nitrite and monosodium glutamate on the histological structure of the kidney in pregnant rats. Twenty-four pregnant rats were used to achieve the aim of the study. The rats were classified into four groups, the first being the control group, the second treated with monosodium glutamate at 10 g/kg, the third injected with sodium nitrite at 115 mg/kg, and the fourth for interaction between the two substances and for the same concentrations. The results showed the occurrence of many lesions in the kidneys of experimental groups rats. The second group included interstitial tissue hyperplasia and necrosis of the glomeruli, infiltration of inflammatory cells, congestion of blood vessels, hydropic degeneration of some tubules, and necrosis of some of them. The third group included congestion, hemorrhage in the pulp area, degeneration of some urinary tubules, necrosis, and deformation of the glomerulus. However, degeneration of some tubules and necrosis were seen in the fourth group, such as glomerulus hyperplasia, reduction of Bowman's space, an increase in acidity of the cytoplasm of epithelial cells tubules, hyperplasia of the fibroblasts, and the desquamation of some tubules. The study concluded that these substances have harmful effects on the kidneys in pregnant rats, especially when they are overlapped, so they must be avoided during pregnancy to maintain kidney health.

Current study tackled potency of MSG and NaNO2 to induce histopathological changes in eye structure of Swiss-mice and protective role of grape seed-oil against toxicity of these substances may cause.In this study, 36 adult mice divided into six groups with control. One group was dosed with MSG at 9g/kg; another was injected with NaNO2 at 110mg/kg for two months, groups with interference of two substances, and two groups with interference of grape seeds-oil with each substance.Results showed emergence of histopathological lesions. In treatment with MSG, there was wide destruction and damage to photoreceptor cells and outer, inner nuclear layer, separation corneal stroma and gliosis in optic nerve. In treatment with NaNO2, damage was extensive in components of eye; it was noted the appearance of roseat pattern in outer, inner nuclear layer, necrosis of outer plexus layer and contraction of lens fibres. When treating with an interference of MSG and NaNO2, damage was observed to the surface epithelial tissue of cornea, stroma fibres, reduction in fibroblasts, necrosis in some cells of ciliary body, and in retina extensive damage was observed in its layers.When treating by MSG with oil, increase in the inner plexus vasa, nerve fibres, and the inner nuclear layer was observed and slight damage to the outer pieces of the photoreceptors. When treating with NaNO2, oil, infiltration of inflammatory cells appeared in the ganglion cells, increased vasa, edema and hyperplasia in the epithelium of the lens.