Coenzyme Q (ubiquinone or Q) is a crucial mitochondrial lipid required for respiratory electron transport in eukaryotes. 4-Hydroxybenozoate (4HB) is an aromatic ring precursor that forms the benzoquinone ring of Q and is used extensively to examine Q biosynthesis. However, the direct precursor compounds and enzymatic steps for synthesis of 4HB in yeast are unknown. Here we show that para-aminobenzoic acid (pABA), a well known precursor of folate, also functions as a precursor for Q biosynthesis. We suggest a mechanism where Schiff base-mediated deimination forms DMQ 6 quinone, thereby eliminating the nitrogen contributed by pABA. This scheme results in the convergence of the 4HB and pABA pathways in eukaryotic Q biosynthesis and has implications regarding the action of pABAbased antifolates. Coenzyme Q (Q)2 is an essential polyprenylated benzoquinone lipid in cellular energy metabolism. The prenyl tail anchors Q in cellular membranes, whereas the redox chemistry of the benzoquinone ring plays a crucial role in respiratory electron transport, in catabolic and biosynthetic metabolism (1), as a co-antioxidant able to recycle vitamin E, and as a chain-terminating antioxidant (2). In these reactions the quinone ring of Q thus cycles between oxidized and reduced (QH 2 , or hydroquinone) states.Cells rely on de novo synthesis for an adequate supply of Q. Studies in Escherichia coli, Schizosaccharomyces pombe, and Saccharomyces cerevisiae have made use of Q-deficient mutants to elucidate the biosynthetic pathway (3-5). In S. cerevisiae, nine COQ genes are required, and each of the yeast coq mutants (coq1 through coq9) lack Q 6 and are unable to grow on media containing non-fermentable carbon sources such as glycerol or ethanol. The dedicated precursors in the biosynthesis of Q are polyisoprene diphosphate, which provides the tail (S. cerevisiae synthesizes Q 6 , with a tail containing six isoprene units), and 4-hydroxybenzoic acid (4HB) (6, 7). Studies in animal cells and in E. coli indicate that different metabolic pathways are used to produce 4HB. Animals (e.g. rats and humans) generate 4HB from the essential dietary amino acid tyrosine (6 -8). Phenylalanine also acts as a precursor for 4HB, however, the incorporation is thought to proceed primarily following its conversion to tyrosine via phenylalanine hydroxylase (8). The biosynthetic steps leading from 4-hydroxyphenylpyruvate to 4HB in animal cells are not yet characterized (see Fig. 1). E. coli relies on shikimate biosynthesis, the formation of chorismate, and chorismate pyruvate lyase (encoded by the ubiC gene) to synthesize 4HB (9, 10). E. coli ubiC mutants lack Q unless 4HB is provided in the growth media (9). E. coli mutants lacking shikimate or chorismate also require exogenous 4HB to synthesize Q (11). Thus, E. coli cells are unable to convert tyrosine or phenylalanine to Q and rely exclusively on the de novo synthesis of 4HB from chorismate.In contrast, S. cerevisiae may utilize either shikimate or tyrosine to synthesize the aromatic ring precursor of Q (6,...
Respiratory electron transport in the plasma membrane of prokaryotes and in the inner mitochondrial membrane of eukaryotes is supported by the crucial lipid component, ubiquinone (coenzyme Q or Q). Qn is composed of a fully substituted benzoquinone ring and a polyisoprene tail of n units. The best‐characterized ring precursor, 4‐hydroxybenozoate (4HB), is derived from the chorismate biosynthetic pathway. Extensive work has shown that the ring carbons of 4HB are incorporated into Q6 in yeast, under the activity of an enzyme complex found in the yeast mitochondrial matrix. However, the direct precursor compounds and enzymatic steps for the generation of 4HB in yeast are unknown. Our recent findings show that para‐aminobenzoic acid (pABA) is also a precursor for Q biosynthesis. In yeast pABA is synthesized from chorismate in two steps: Abz1 amidates chorismate to form 4‐deoxyaminochorismate, and Abz2 is a lyase that forms pABA. Here we show that the production of a prenylated form of pABA is dependent on the presence of the ABZ1 gene. We also demonstrate by LC‐MSMS analyses that yeast incubated with stable isotope forms of [13C6‐ring]‐pABA or [13C6‐ring]‐4HB generate [13C6]‐coenzyme Q6. These results identify a novel biosynthetic pathway for the production of Q in yeast. This research was supported by grants from the NIH GM 45952, NSF MCB‐0919609, and by a grant S10RR024605 from the National Center for Research Resources.
which all functional groups except one are protected, are prepared via Kiliani reaction of mannose and gulose ketals. Heptonolactones have been used as starting materials for the preparation of piperidines such as homomannojirimycin, castanospermines and mannostatins. -(BEACHAM, A. R.; BRUCE, I.; CHOI, S.; DOHERTY, O.; FAIRBANKS, A. J.; FLEET, G. W. J.; SKEAD, B. M.; PEACH, J. M.; SAUNDERS, J.; WATKIN, D. J.; Tetrahedron: Asymmetry 2 (1991) 9, 883-900; Dyson Perrins Lab., Oxford Cent. Mol. Sci., Oxford OX1 3QY, UK; EN)
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