On a global scale, wheat (Triticum aestivum L.) is a widely cultivated crop among all cereals. Increasing pollution, population expansion, socio-economic development, ecological and industrial policies have induced changes in overall climatic attributes. The impact of these factors on agriculture dynamics has led to various biotic and abiotic stresses, i.e., significant decline in rainfall, directly affect sustainable agriculture. Increasing abiotic stresses have a direct negative effect on worldwide crop production. More promising and improved stress-tolerant strategies that can help to feed the increasing global population are required. A laboratory experiment was performed on two of the latest wheat (Triticum aestivum L.) genotypes (Akbar 2019 and Anaj 2017) from Punjab Pakistan, to determine the influence of seed priming with thiamine (vitamin B1) along with soil inoculation of Endophytic bacterial strains to mitigate the effects of drought stress at different degrees. Results revealed that thiamine helped in the remote germination; seeds of Anaj 2017 germinated within 16 hours while Akbar 2019 germinated after one day. Overall growth parameters of Anaj 2017 were negatively affected even under higher levels of drought stress, while Akbar 2019 proved to be a susceptible cultivar. A significant increase in RFW (54%), SFW (85%), RDW (69%), SDW (67%) and TChl (136%) validated the effectiveness of D-T3 compared to C-T0 in drought stress. Significant decrease in MDA, EL and H2O2 signified the imperative function of D-T3 over C-T0 under drought stress. In conclusion and recommendation, we declare that farmers can get better wheat growth under drought stress by application of D-T3 over C-T0.
Drought stress and poor zinc (Zn) are major constraints for commercial agriculture. Their detrimental effects significantly decrease crop’s growth and yield. Less water uptake disturbs the metabolic processes in plants. However, the deficiency of Zn leads to the inactivation of many enzymes. It is well documented that cereal crops, especially wheat, are susceptible to drought and Zn deficiency. Scientists suggest the supplementation of Zn along bio-fertilizers for the sustainable management of these issues. That is why the current experiment was conducted to explore the best combination of Zn and bio-fertilizer for wheat. There were two different recommended concentrations of Zn sulfate (Zinc level 1 (Zn1) = 20 and Zinc level 2 (Zn2) = 40 kg ha−1) applied under normal irrigation (75% field capacity = FC) and severe drought stress (40% FC). Sole and combined inoculation of arbuscular mycorrhizal fungi (AM) with Zn1 and Zn2 was also performed. Osmotic stress (40% FC) significantly decreased the examined growth parameters. It also significantly enhanced antioxidant and oxidative indicators in wheat. A significant increase in root fresh weight, root dry weight, and shoot length while a significant decrease in EL, SOD, POD over the control validated the efficacious role of Zn2 + AM. It is concluded that Zn2 + AM can improve wheat root fresh weight and root length wheat under 40% FC. Under different climatic zones, wheat varieties, and soil types, more investigations are recommended to declare Zn2 + AM as the best amendment for improving wheat growth attributes under osmotic stress.
Biochar, prepared from organic waste materials, can improve the quality of contaminated soil areas. Biochar can be used as an economic centerpiece over other available resources and can properly utilize large amounts of waste. Soil contaminated with cadmium (Cd) is a worldwide problem that poses potential agricultural and human health hazards. Moreover, Cd toxicity causes serious problems for sustainable food production, especially in food crops like barley. High cadmium concentration in soil is phytotoxic and decreases plant growth and ultimately yields. Biochar and ascorbic acid in ameliorating Cd stress are economically compatible and consistent approaches in agriculture. The present study aimed to evaluate biochar’s and foliar-applied ascorbic acid’s influence on some growth and biochemical characteristics of barley (Hordeum vulgare L.) to Cd stress. The soil was supplemented with biochar 2% w/w and 20 mg Cd kg−1. The foliar application of 30 mM ascorbic acid was done on plants. The results revealed that Cd stress decreased chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids. It also increased oxidative stress indicators, i.e., APX, COD, POD, flavonoids, anthocyanin, phenolics, and electrolyte leakage, in barley with Cd-contamination. A significant enhancement in root and shoot length, gas exchange attributes, and chlorophyll contents validated the effectiveness of Bio + Asa treatments over all other treatments under Cd contamination. In conclusion, the sole applications of biochar and Asa in Cd contamination are also effective, but Bio + Asa is a better amendment for Cd stress alleviation in barley plants.
Arsenic (As) contamination is a serious threat to agriculture and human health worldwide. It can adversely affect the growth attributes of food crops. On the other hand, using thiourea (TU) to ameliorate As stress is an economically consistent approach. However, there is a knowledge gap regarding the combined use of TU and Sewage sludge (SS). SS is considered important, unutilized biomass. It can be used as a fertilizer that has high organic matter and nutrients. Therefore, the current study was performed to evaluate TU and SS sole and combined responses under As toxicity on two wheat genotypes (Markaz 19 and Ujala 16). There were four treatments control (As 50 mg kg-1), SS (30 g kg−1)+TU (6.5 mM)+As, TU+As and SS+As applied with four replications. Results revealed that SS+TU performed significantly better over SS, TU and control for improvement in root and shoot fresh and dry weight of wheat varieties Markaz 19 and Ujala 16 under As toxicity. A significant decrease in POD, SOD and APX of Markaz 19 and Ujala 16 also validated the effective functioning of SS+TU over control. The maximum increase of 71 and 77% was noted in phosphorus, where SS+TU was applied over control in Markaz 19 and Ujala 16, respectively. In conclusion, SS+TU is a better approach than the sole application of SS and TU under As contamination for improvement in wheat growth attributes. More investigations are recommended at the field level under different As contamination and agro-climatic zones to declare SS+TU an effective amendment to mitigate As toxicity in wheat.
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