Pot experiment was carried out during May 2019 at private farm in el Manyial, Cairo, Egypt. The investigation target was to find out the difference between using plant growth promoting rhizobacteria (B. cereus, Streptomyces chibaensis, B. megaterium, B. polymyxa) either individual or in combination with each other and mineral fertilization on antimicrobial activity and some phytochemical constitutes of Moringa olifera leaves. The highest values of antimicrobial activity recorded for mineral fertilization on treatment followed by mixed biofertilization treatment (GP4) (B. megaterium, B. polymyxa) against seven foodborne pathogens Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus niger, Rhizopus stolonifer and Botrytis cinera, as compared with the control treatment. Biofertilization treatment (B. megaterium, B. polymyxa) recorded increasing over control in (GP10) by 160.8% and decreasing of chemical fertilization by 5.41% for polyphenol content, also, the antioxidant content shown increasing over the control (GP10) by 81.1% and decreasing of chemical fertilization (GP9) by 5.6% as well as vitamin C and A were increased with mineral and biofertilizer (GP4) treatments after 55 days. Biofertilization in (GP4) recorded increasing over control group (GP10) by 48.89%, 27.08% and 58% for vitamin A, C and chlorophyll respectively. Ethyl acetate extract was found to be most potent extract than ethanol and water against all tested microorganism.
Aerated Compost Tea (ACT) prepared from four types of compost; Agricultural Residues(AR), Agricultural Residues enriched with Olive Pomace (AR+OP), Municipal Solid Wastes (MSW) and Municipal Solid Wastes enriched with OP(MSW+OP) in combination with bioagents have been analytically characterized and were tested for their antagonistic activity in vitro against Fusarium oxysporum and Rhizoctonia solani pathogens in comparison with the commercial chemical fungicides (Rhizolex-T). Assessment was carried out in terms of percent mycelial growth inhibition as well as Scanning Electron Microscopy (SEM) were used to explain the mode of action in the biological control of the pathogens under study. From the resultsall compost teas showed high levels of nutrients and microbial biomass content. The mixed treatment of compost tea gave the highest percent inhibition compared with individually treatments. AR compost tea+ Olive Pomace (OP)+ Microorganisms (MO) were significantly superior to the rest treatments showed 63.52% and 44.07% percent inhibition, same trend was detected with MSW+ Olive Pomace (OP)+ Microorganisms (MO) showing 57.41% and 43.89% against Rhizoctonia solani andFusarium oxysporumrespectively. SEM of tested pathogen mycelium removed from the confrontation zone showed aberrant morphology such as shrinkage, curling,mycelium asymmetry, partial distortions and lysis of fungal mycelium. In conclusion, these results provide evidence that the compost extract has the potential to become a good candidate for biological control as potential alternatives to the application of synthetic fungicides, and as plant promoters in crop production, for attaining environmental sustainability for farming and food safety.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.