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During present study a rapid in vitro propagation method was developed for Inula royleana, a medicinal perennial herb, through embryo culture. Embryo germination and complete plantlet formation was obtained on basal MS medium in a time period of 6 and 11 days respectively with 100% culture response. Indirect shoot regeneration with 25±0.6 mean number of shoots was also obtained in 70% cultures when leaf explants obtained from germinated embryos were inoculated on MS medium containing BAP (5 mg/l) +IAA (2 mg/l).

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Effects of cytokinins were studied on multiple shoot regeneration in Inula royleana DC. Callus from leaf of I. royleana was treated with different concentrations of BAP and in order to find out the limiting concentrations of both BAP and Kn at which maximum shoot regeneration took place. Among different concentrations 1 mg/l BAP and 0.8 mg/l Kn proved to be optimum. Rooting was obtained in White’s medium. Regenerated plantlets were successfully acclimatized in pots containing vermicompost.Plant Tissue Cult. & Biotech. 27(2): 189-194, 2017 (December)