Pdx1 exon2 (Figure 4) through pancreatic duct infusion into the pancreas.RESULTS AND CONCLUSIONS: These CRISPR-SaCas9 and gRNAs AAV viruses were co-infused into pancreases of C57BL/6J mice 2 days after alloxan treatment. One week after AAV6-SaCas9+gRNAs infusion, it appeared that only the Pdx1 in pancreatic ductal cells were deleted. Comparatively, PBS or AAV6-CMV-GFP viral infused C57BL/6J mice and ROSA26 LSL tomato reporter mice expressed fl uorescence in the entire pancreas as a result of the deleted loxP sites that removed the stop cassette, the Pdx1 is not deleted. Thus, we fi rst report here a highly effi cient and specifi c ablation of Pdx1 in adult mouse pancreatic ducts using the CRISPR-Cas9 technique.Figure 1.Construction of AAV CRISPR-SaCas9 and gRNA vectors. Figure 2. Deletion of ZsGeen in HEK293-CMV-GFP cells using SacCas9 and gRNA in vitro. Figure 3. Valiation of SaCas9 and gRNA in vivo deletion of loxP sites in ROSA26-LSL tomato mice pancreas. Figure 4. Deletion of Pdx1 exon2 in alloxan treated C57 pancreatic ducts.
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