Antimicrobial resistance is an increasing issue in healthcare as the overuse of antibacterial agents rises during the COVID-19 pandemic. The need for new antibiotics is high, while the arsenal of available agents is decreasing, especially for the treatment of infections by Gram-negative bacteria like Escherichia coli. Antimicrobial peptides (AMPs) are offering a promising route for novel antibiotic development and deep learning techniques can be utilised for successful AMP design. In this study, a long short-term memory (LSTM) generative model and a bidirectional LSTM classification model were constructed to design short novel AMP sequences with potential antibacterial activity against E. coli. Two versions of the generative model and six versions of the classification model were trained and optimised using Bayesian hyperparameter optimisation. These models were used to generate sets of short novel sequences that were classified as antimicrobial or non-antimicrobial. The validation accuracies of the classification models were 81.6–88.9% and the novel AMPs were classified as antimicrobial with accuracies of 70.6–91.7%. Predicted three-dimensional conformations of selected short AMPs exhibited the alpha-helical structure with amphipathic surfaces. This demonstrates that LSTMs are effective tools for generating novel AMPs against targeted bacteria and could be utilised in the search for new antibiotics leads.

The rising popularity of autonomous vehicles has led to the development of driverless racing cars, where the competitive nature of motorsport has the potential to drive innovations in autonomous vehicle technology. The challenge of racing requires the sensors, object detection and vehicle control systems to work together at the highest possible speed and computational efficiency. This paper describes an autonomous driving system for a self-driving racing vehicle application using a modest sensor suite coupled with accessible processing hardware, with an object detection system capable of a frame rate of 25fps, and a mean average precision of 92%. A modelling tool is developed in open-source software for real-time dynamic simulation of the autonomous vehicle and associated sensors, which is fully interchangeable with the real vehicle. The simulator provides performance metrics, which enables accelerated and enhanced quantitative analysis, tuning and optimisation of the autonomous control system algorithms. A design study demonstrates the ability of the simulation to assist in control system parameter tuningresulting in a 12% reduction in lap time, and an average velocity of 25 km/h -indicating the value of using simulation for the optimisation of multiple parameters in the autonomous control system.

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The widespread development of driverless vehicles has led to the formation of autonomous racing competitions, where the high speeds and fierce rivalry in motorsport provide a testbed to accelerate technology development. A particular challenge for an autonomous vehicle is that of identifying a target trajectory -or in the case of a racing car, the ideal racing line. Many existing approaches to identifying the racing line are either not the time-optimal solutions, or have solution times which are computationally expensive, thus rendering them unsuitable for real-time application using on-board processing hardware. This paper describes a machine learning approach to generating an accurate prediction of the racing line in real-time on desktop processing hardware. The proposed algorithm is a dense feed-forward neural network, trained using a dataset comprising racing lines for a large number of circuits calculated via a traditional optimal control lap time simulation. The network is capable of predicting the racing line with a mean absolute error of ±0.27m, meaning that the accuracy outperforms a human driver, and is comparable to other parts of the autonomous vehicle control system. The system generates predictions within 33ms, making it over 9,000 times faster than traditional methods of finding the optimal racing line. Results suggest that a data-driven approach may therefore be favourable for real-time generation of nearoptimal racing lines than traditional computational methods.