The purpose of this study was to investigate the effects of different irrigation solutions on the smear layer removal and marginal adaptation of a resin-based sealer to root canal dentine. A total of 152 instrumented roots were irrigated with the following irrigants: 9,18% etidronic acid (HEBP), 0.5, 1,2% peracetic acid (PAA), 17% ethylenediaminetetraacetic acid (EDTA), saline. The amount of smear layer was evaluated using scanning electron microscope (SEM) in seventy root samples.Eighty-two roots were filled with AH Plus and gutta-percha. Slices obtained from apical third of each specimen were viewed with SEM to assess marginal adaptation. Use of 9% and 18% HEBP resulted in more efficient smear layer removal in the apical third than the other chelators (p < 0.05). Higher smear layer scores in the coronal and middle thirds were obtained from 0.5%, 1% PAA groups. Regarding marginal adaptation, 18% HEBP group showed the lowest gap size values (p < 0.05), and better marginal adaptation. Etidronic acid is a promising candidate for final irrigation of root canals.

This study evaluated the effects of 9% etidronic acid (HEBP) and 17% ethylenediaminetetraacetic acid (EDTA) used either with conventional syringe irrigation (CSI) or passive ultrasonic irrigation (PUI) on the fracture resistance of roots with different dentine thickness. The root canals of 120 extracted teeth were widened, leaving dentinal walls with different thicknesses of either 0.75 mm, 1.50 mm, or 2.25 mm with 40 teeth in each group. Within each of these groups, four subgroups (n = 10) were formed according to the final irrigation regimens applied: 2.5% NaOCl‐17% EDTA with CSI; 2.5% NaOCl‐17% EDTA with PUI; 2.5% NaOCl + 9% HEBP with CSI; 2.5% NaOCl + 9% HEBP with PUI. The irrigated root canals were obturated with AH Plus sealer and gutta‐percha cones. A compressive vertical loading was applied to the samples and the force at fracture was recorded. Data were statistically analysed using a multiple linear regression analysis. The most influencing factor for the fracture strength of roots was the remaining dentine thickness, followed by the irrigation method, and then the irrigation solution. The samples irrigated using PUI were statistically significantly more resistant to fracture than those using CSI (mean difference = 116.3 N; 95% CI = [53.9, 178.6] N). The application of HEBP was associated with higher resistance to fracture than the application of EDTA (mean difference = 71.0 N; 95% CI = [8.6, 133.3] N).