In Jordan there are different environments and altitudes that have an effect on olive oil properties and fatty acid composition, which therefore affect its quality. Taking into account that there are no previous similar studies in this field, this study investigates the ratios and composition of the main fatty acids in mixed olive oil from different areas in Jordan (above and below the sea level); namely the Jordan Valley, Subaihi, Kufranja and Madaba areas where the elevation ranges between (-230 m) below sea level and (+785 m) above sea level. The study measures the impact of the location of olive orchards on the fatty acid composition of olive oil and of its quality. Three olive oil samples were collected from the “Nabali Muhasan” and “Baladi” varieties from each area. They were analyzed in the National Agriculture Research Center (NARC) laboratories using GAS Chromatography. The results showed that the ratios of fatty acids are significantly affected by the degree of above elevation of the olive trees sea level. This confirms the impact of the geographical and climatic environment, which is reflected on the olive oil quality due to differences in the ratios of fatty acids in the four planting areas. This shows that the mean concentration of the fatty acids in the olive oil in these four planting areas is not equal. In general, there is a significant difference in the fatty acid concentrations compared to the standard concentration because of the planting location of olive trees, given that olive oil fatty acids in each location work together in a balanced integrative relationship.
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