Abstract. There is a limited number of reports regarding detergents and proteases inactivating, degrading, or destabilizing abnormal prion protein (PrP Sc ). In the present study, the effect of alkaline detergents and proteases on the breakdown of PrP Sc in the absence of proteinase K (PK) (degradation) and the presence of PK (destabilization) was investigated. PrPSc from brain homogenate of terminallydiseased mice infected with the Chandler strain of scrapie was used as a substrate. A surfactant-free alkaline detergent (pH 11.9, 1% aqueous solution) with potassium hydroxide as the main ingredient and an alkaline detergent (pH 11.9, 1% aqueous solution) containing about 1% surfactant as well as two commercially available alkaline proteases had a destabilizing effect on PrP Sc . All these detergents and proteases showed degradative effects on PrP Sc under appropriate conditions. These results demonstrate the usefulness of alkaline detergents and proteases for the degradation or destabilization of PrP Sc .
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