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At the current work, the substrate phenyl hydrazine reacted with selected thiophene-2-carbaldehyde in absolute ethanol and a few drops of glacial acetic acid to give 1-phenyl-2-(thiophen-2-ylmethylene)hydrazine (PTMH) as a selective and sensitive reagent. It was synthesized and studied to determine cobalt spectroscopy in the following detail:  at a pH value of 6.0, which greatly increased the selectivity, Co(II) reacted with (PTMH) to form a 1:2 complexes, with absorption peaks at 386 nm for cobalt. Under the optimal conditions, Beers law was obeyed over the ranges of (0.14ـــ5.8) mg/L. The apparent molar absorptivity and Sandell’s  sensitivities were 7.1360×104 L.mol-1.cm-1, and 8.2585×10-4 µg.cm-2. The detection limits were found to be 0.0133 and the limit of quantification is 0.0466.

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Through this study, nanocomposite co-polymer was prepared by reaction between Pentaerythritl and phthalic anhydride. Infrared spectroscopy and AFM technique was used for the purpose of distinguishing the nanopolymer. Thus, the ability of the synthetic polymer to absorb the Orange-G pigment present in aqueous solutions was found. It was found that the nanopolymer had an average height of 5.26 nm. Therefore, three different temperatures were used (298, 308 and 318 K). Also Three concentrations of part units per million (1,3 and 5) of the nanopolymer synthesized were used for the study. Through this study, it was found that the nanopolymer is effective in removing Orange-Gfrom all aqueous solutions.