Objective: The objectives of this study were to evaluate the compliance of hand hygiene among dental health care professionals, to access different variables associated with hand hygiene and to identify possible ways of improving hand hygiene compliance. Methods: This descriptive cross-sectional study was done to access the hand hygiene compliance, knowledge and practice of dental house officers in public dental institutes using a questionnaire developed by the authors. Descriptive statistics and chi square test were performed and a P value <0.05 was taken as significant. Results: 88 dental house officers working in different clinical departments participated in this study. 90% of the respondents believed that hand hygiene plays an important role in cross infection control. More than two-third were aware of all the steps recommended for hand washing, but more than half were not following them. 60% of the respondents wash their hands before and after touching each patient. However, 30% wash hands after each patient and 10% only wash hands before touching every patient. Fifty-five (55.8%) of the participants routinely use alcohol-based rub for hand hygiene. There was a significant difference regarding hand hygiene training among different departments. (p<0.001) Majority of house officers working in prosthodontics and surgery had not received hand hygiene training. Conclusion: Hand hygiene compliance is found to be acceptable when compared with developing countries. However, there was lack in knowledge. Dental graduates should be made aware of CDC and ADA guidelines. Better facilities and instruction for dental students should be encouraged. Hand hygiene quality needs to be improved on an urgent basis. Keywords: Hand Hygiene, Dental Health, Hand Washing, Bacteria
Pulp-Dentin regeneration is a key aspect of maintain tooth vitality and enabling good oral-systemic health. This study aimed to investigate a nanofibrous scaffold loaded with a small molecule i.e. Tideglusib to promote odontogenic differentiation. Tideglusib (GSK-3β inhibitor) interaction with GSK-3β was determined using molecular docking and stabilization of β-catenin was examined by confocal microscopy. 3D nanofibrous scaffolds were fabricated through electrospinning and their physicochemical characterizations were performed. Scaffolds were seeded with mesenchymal stem cells or pre-odontoblast cells to determine the cells proliferation and odontogenic differentiation. Our results showed that Tideglusib (TG) binds with GSK-3β at Cys199 residue. Stabilization and nuclear translocation of β-catenin was increased in the odontoblast cells treated with TG. SEM analysis revealed that nanofibers exhibited controlled architectural features that effectively mimicked the natural ECM. UV-Vis spectroscopy demonstrated that TG was incorporated successfully and released in a controlled manner. Both kinds of biomimetic nanofibrous matrices (PCLF-TG100, PCLF-TG1000) significantly stimulated cells proliferation. Furthermore, these scaffolds significantly induced dentinogenic markers (ALP, and DSPP) expression and biomineralization. In contrast to current pulp capping material driving dentin repair, the sophisticated, polymeric scaffold systems with soluble and insoluble spatiotemporal cues described here can direct stem cell differentiation and dentin regeneration. Hence, bioactive small molecule-incorporated nanofibrous scaffold suggests an innovative clinical tool for dentin tissue engineering.
Objective: The present study aimed to evaluate the level of perceived stress is associated with self-reported halitosis among undergraduate dental students in Pakistan Method: The crossectional study based on survey design was directed to evaluate the association of level of perceived stress and halitosis among undergraduate dental students. Sample size of the study was 278 undergraduate dental students from private dental colleges of Lahore. Independent sample t test was used. Results: The results of independent sample t test revealed a significant difference of level of perceived stress between the undergraduate dental students with halitosis and without halitosis (t=-21.784, P=.000). Undergraduate dental students who don’t have reported halitosis have lower level of perceived stress (Mean±SD, 11.76±3.01) as compared to those students who reported halitosis (Mean±SD, 22.13±4.32) Conclusion: Higher prevalence of halitosis was found among dental students with moderate perceived stress Keywords: Halitosis, Dental students, Perceived stress
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