No abstract

Forests across the world are considered to be a huge socio-economic and environmental benefit to host and adjacent communities. This study focuses on assessing the impacts of fuelwood and timber consumption on the livelihood of households in the Baltistan region in Pakistan. Primary and secondary sources of data were employed for the study. The primary sources involved the use of questionnaire survey and interview while the secondary sources involved the use of documented information in textbooks and internet materials. The study revealed that 82% of the people within the region were involved in agricultural activities, 71% depended on the extraction of forest resources for their livelihood, while 18% depended on off-farm activities for their livelihood. The study also observed that among the number that depended on forest resources for their livelihood, 59% were involved in the extraction of non-timber forest products while 41% were involved in the extraction of timber forest resources. The study further revealed that there was no significant difference in the level of benefits from the forest across the seven districts under investigation with a chi square value. The volume of forest products extraction was found to be high closest to the forest and to be low with increasing distance from the communities. The major benefits from the forest range were due to employment that increases the individual and family income. Forest also helps to control erosion and enhances aesthetic beautification and temperature regulation. The research suggests that the policy makers must provide a sustainable solution to reduce the overexploitation of the forest resources by providing better alternative earning resources to the resident communities.

Artemisia absinthium L. is a highly medicinal plant with a broad range of biomedical applications. A. absinthium callus cultures were established in response to bio-fabricated single NPs (Ag and Cu) or a combination of both NPs (Ag and Cu) in different ratios (1:2, 2:1, 1:3, and 3:1) along with thidiazuron (TDZ) (4 mg/L) to elicit the biomass accumulation, production of non-enzymatic compounds, antioxidative enzymes, and antioxidant activity. Silver and copper nanoparticles (Ag and Cu NPs) were synthesized using the leaves of Moringa oleifera as reducing and capping agent and further characterized through UV-Visible spectroscopy and SEM. The 30 µg/L suspension of Ag and Cu NPs (1:2, 2:1) and 4 mg/L TDZ showed 100% biomass accumulation as compared to control (86%). TDZ in combination with Ag NPs enhanced biomass in the log phases of growth kinetics. The Cu NPs alone enhanced the superoxide dismutase activity (0.56 nM/min/mg FW) and peroxidase activity (0.31 nM/min/mg FW) in callus cultures. However, the combination of Ag and Cu NPs with TDZ induced significant total phenolic (7.31 µg/g DW) and flavonoid contents (9.27 µg/g DW). Furthermore, the antioxidant activity was highest (86%) in the Ag and Cu NPs (3:1) augmented media. The present study provides the first evidence of bio-fabricated single NPs (Ag and Cu) or a combination of both NPs (Ag and Cu) in different ratios (1:2, 2:1, 1:3, and 3:1) along with TDZ (4 mg/L) on the development of callus culture, production of endogenous enzymes, non-enzymatic components, and further antioxidant activity in callus cultures of A. absinthium.

Ginkgo biloba L. is a well-known tree for its high medicinal value owing to the presence of terpene trilactones, unique active metabolites, and other phytochemicals, including flavonoids and phenolics in its leaves. In Egypt, G. biloba has been reported as one of the endangered trees. Hence, plant tissue technique could be a potential alternative system to produce ginkgo's pharmaceutical compounds. In this study, an elicitation strategy using the biotic elicitor chitosan was employed to enhance the productivity of certain metabolites in G. biloba leaf-derived callus incubated in light (16 h photoperiod) or darkness. Combination of 1.0 mg/L α-naphthalene acetic acid (NAA) and 2.0 mg/L 6-benzyl adenine (BA) added to Murashige and Skoog (MS) medium resulted in the highest callogenesis percentage in both light and dark (95.00 and 94.43%, respectively) with a compacted callus texture but a different color. Significant differences were recorded between chitosan levels concerning callus biomass production and total flavonoids and phenolics accumulation. The highest yield of callus fresh and dry weights, growth index, and relative growth rate were recorded for light-grown cultures elicited with 100 mg/L chitosan followed by 50 mg/L. Same concentrations were also superior in enhancing the content and productivity of total flavonoids and total phenolics. Light incubation was more favorable than darkness for callus growth, flavonoid, and phenolic biosynthesis as well as antioxidant activity. The current study revealed the possibility of using calli culture as a biotechnological approach for the industrial production of G. biloba phytoconstituents.

Forest utilization makes a significant economic contribution to the livelihoods of rural households, especially those living in developing countries. This study was conducted to determine the absolute and relative forest income and measure the distributional impact of forest income on economic inequalities among rural households in the Rubi-Tele Hunting Domain (RTHD), Democratic Republic of the Congo (DR Congo). Household socioeconomic data and other qualitative informations were collected using structured household-level surveys, focus group discussions, and key informant interviews. The study used descriptive statistical analysis, Gini coefficient, and Lorenz curve computation. Additionally, the non-parametric alternative Kruskal–Wallis Test was also used. Findings revealed that 89.76% of the households’ sample were involved in forest-based activities. The annual average income from these forest-based activities was estimated at CDF 1,219,951.58 (USD 659.08) per household, contributing 32.46% to total annual household income. Other income sources share constituted 37.09%, 10.04%, 8.30%, 7.63%, 2.41%, and 2.08% from crop, livestock keeping, business activities, fishing, and non-forest environmental activities, paid employment, and other income sources, respectively. There was a significant difference between annual household income in terms of income sources (p < 0.5). Forest income constituted the second most crucial income portfolio after crop income. Bushmeat income share was higher than other forest products income share. However, the proportion of households involved in firewood extraction was substantial (80.70%). Gini coefficient and Lorenz curve analysis proved that forest income helped reduce economic inequality among the sampled households by 11%. Therefore, reducing access to forest would significantly impact rural households’ welfare and could increase income disparities. The present study provides valuable information for developing sustainable forest management policies and strategies to maintain and enhance the economic benefit of forest use without damaging biodiversity conservation.