Metformin is an oral anti-diabetic drug in preventing complications of type 2 diabetes and it is a good first-line therapy for an over-obese with type 2 diabetes, it is currently available in more than 60 countries worldwide. As a result of the importance of this oral hypoglycaemic agent in the treatment of non-insulin-dependent diabetes mellitus, which leads to end-stage renal disease, this work aims to compile the published analytical methods reported so far in the literature for the determination in biological samples and pharmaceutical formulations. This article narrates different techniques like high-performance liquid chromatography. It can be seen that high-performance liquid chromatography methods have been used extensively. Thus, this paper will help in the selection and development of proper analytical methodologies estimation of Metformin to achieve satisfactory results.
The onslaught of pathogens (disease-causing microbes) like bacteria and mold is responsible for the majority of cases of food deterioration. The various techniques for food preservation that have been developed are all intended to lessen or completely get rid of harmful ingredients. The term "food preservation" refers to methods for preserving food. Food preservative improves human health by eradicating or restricting the growth of bacteria and organisms responsible for food deterioration. There has been an increase in recent decades in the consumption of foods with high preservative content. By using several analytical techniques, including Uv-Visible, Calorimetry, HPLC, GC, LCMS, and Electrophoresis, the proposed methods were employed to identify various preservatives in a variety of food products.
A protocol is used to detect and measure biomolecules and metabolites in human and animal tissues using bimolecular methods. The biosanalinity method is effective at determining the number of drugs and metabolites in a biological system. New methods, the validation of existing procedures, and the analysis of samples are one of the prominent tasks for bioanalysis. Above all, a compound can be measured using several methods and identified by different methods of analysis. Drugs may be tested by several extraction techniques, including liquid extraction, solid-phase extraction, and protein precipitation in complex plasma and biological samples. To determine how the environment, matrix, or procedures impact the matrix estimation to the time of the analysis, all steps in the process must be investigated. The more detailed study of drug products can be performed with higher-pressure analytical techniques, such as high- extraction (HPLC), liquid chromatography coupled with double-mass spectrometry (LCMS/MS), and ultra-performance Liquid chromatography (UPLC). Both of them have flaws and strengths. At present, HPLC and GC usually perform biolysis. The parameters are linearity, repeatability, accuracy, selectivity, and continuity. We are proposing the development and validation of bioanalytical systems to assist in the quality assurance of drugs.
The bioanalytical analysis of anticancer agents established a more personalized treatment procedure. The importance of validating analytical procedures before they are put into normal usage is widely acknowledged. This novel approach has a lot of promise because it is quick, easy, and only requires a little amount of samples to get the accurate result. The goal of this mini review is to provide a comparative analysis of contemporary research on few anticancer agents and their methodology in reference to bioanalytical analysis. We provide practical approaches for determining extraction and clean up, precision and accuracy, selectivity and specificity, chromatographic analysis and its validation. We believe that the liquid chromatographic processes used in the bioanalysis of anticancer medicines, validation standards might have been applied in a variety of ways to counter the failure of an anticancer agent by increasing its therapeutic index approach.
This study was set out to create an RP-HPLC system that is effective, sensitive, picky, precise, accurate and practical. For this, a UV detection technique for detecting Sorafenib tosylate-loaded solid lipid nanoparticles has been developed and validated. To improve the procedure, many parameters were used (pH and Column). The chromatographic separation was carried out using a Shimadzu prominence-i LC-2030C and a C8 short column (5 m 4.6 x 100 mm). With a runtime of 10 minutes, 10 mL injection volume was maintained at 1 mL/min flow rate. The mobile phase used in the study was a mixture of 70:30 methanol:0.1% formic acid in water. The effluent was detected at 261nm using a UV detector. Drug Entrapment Efficiency (DEE) and Drug Loading (DL) for ST from the extracted SLNs matrix were found to be 86.9% and 19%, respectively. The developed analytical method exhibited a linearity range of 1-64g/ml and an R2 value of 0.998. 0.88 g/ml detection limit (LOD) and 1.0 g/ml limit of quantification (LOQ), and 0.88 g/ml detection limit (LOQ). Using ICH Q2(R1) guidelines, the proposed technique was evaluated, and it was shown to be accurate, linear, robust, and specific. Using the devised analytical method, drug release, drug loading, and drug entrapment effectiveness were all studied.
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