A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Oxidative stress has been well recognized as a component in the pathogenesis of many diseases, including female infertility. In this study, we aimed to investigate serum paraoxonase (PON1), arylesterase and myeloperoxidase (MPO) activities, and lipid hydroperoxide (LOOH) levels as oxidative stress markers along with their relationship with the pregnancy rate in women undergoing in vitro fertilization (IVF) treatment. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Serum samples of 179 subjects were collected immediately before starting ovarian stimulation and on ovum pick-up (OPU) day during IVF treatment. Stimulation protocol and starting doses were determined individually. When the leading follicle reached at least 17 mm in size, recombinant human chorionic gonadotropin (Ovitrelle, 250 mcg, Serono) was administered for ovulation induction. Serum basal/salt-stimulated paraoxonase, arylesterase activities, myeloperoxidase activity, and lipid hydroperoxide levels were compared between two samples. Subjects were also divided into two groups according to the presence of pregnancy, and the groups were compared in terms of oxidative stress markers studied. R Re es su ul lt ts s: : Serum MPO activity and LOOH levels were significantly higher on ovum pick up day (p<0.05, p<0.001, respectively), while basal/salt-stimulated paraoxonase and arylesterase activities were significantly lower (p=0.012, p=0.041, respectively) than those before ovarian stimulation. Serum LOOH levels on OPU day were significantly higher in non-pregnant group than pregnant one (p<0.001). Although basal/salt-stimulated paraoxonase, arylesterase and myeloperoxidase activities were higher in the non-pregnant group compared to the pregnant group, it was not statistically significant (p>0.05). C Co on nc cl lu us si io on n: : Ovarian stimulation during IVF treatment resulted in increased oxidative stress and decreased PON1 activity. However, changes in the studied parameters were not found to be significantly associated with the pregnancy results. Further studies are required to clarify our results.K Ke ey y W Wo or rd ds s: : In vitro fertilization; lipid peroxides; peroxidase; PON1 protein, human Ö ÖZ ZE ET T A Am ma aç ç: : Oksidatif stresin, kadın infertilitesi de dahil olmak üzere bir çok hastalığın patogenezinde yer aldığı gösterilmiştir. Bu çalışmada, in vitro fertilizasyon (IVF) tedavisi uygulanan kadın-larda oksidatif stres belirteçlerinden serum paraoksonaz (PON1), myeloperoksidaz (MPO), aril esteraz aktiviteleri ile lipid hidroksiperoksid (LOOH) düzeylerini ve bu belirteçlerin gebelik oranlarıyla ilişkisini araştırmayı amaçladık. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Yüz yetmiş dokuz olgunun serum örnekleri ovaryan stimulasyona başlanmadan hemen önce ve tedavi esnasında yumurta toplama günü (OPU) alındı. Stimulasyon protokolü ve başlangıç dozları bireysel olarak belirlendi. Dominant folikül boyutu en az 17 mm'ye ulaştığında, ovulasyonu tetiklemek için reko...

BACKGROUND: Ischemia-reperfusion injury is one of the leading causes of acute renal failure which is a common clinical event leading to development of chronic kidney disease and a high mortality; especially in elderly people. β-glucans are glucose polymer groups with free-radical scavenger, macrophage activator, and immune defense inducer functions. We designed this study to determine the possible protective effects of β-glucan against renal ischemia-reperfusion injury comparatively in young and aged rats. METHODS: Rats were assigned to the following groups: Young and aged sham, young and aged ischemia-reperfusion, young and aged β-glucan, young and aged ischemia-reperfusion+β-glucan. At the end of the experiment, following collection of blood samples, rats were sacrifi ced and kidneys were removed for histopathological and biochemical examination. RESULTS: Mean tissue histopathological damage scores of young β-glucan group was lower than that of young ischemia-reperfusion group, and of aged β-glucan group was lower than that of aged ischemia-reperfusion group. Urea and creatinine levels of young and aged of sham group and β-glucan administered groups were all lower than those of ischemia-reperfusion and β-glucan+ischemia-reperfusion groups. Oxidative stress indexes of ischemia-reperfusion groups were increased however ; oxidative stress indexes of β-glucan administered to young and aged rats were lower than those of ischemia-reperfusion groups. CONCLUSIONS: We conclude that β-glucan is effective to protect kidneys from ischemia-reperfusion-induced oxidative damage, especially in young rats (Fig. 6, Ref. 45). Text in PDF www.elis.sk.

Son yıllarda yapılan çalışmalara göre Urfa fıstığı (Pistacia vera L.) fenolik bileşiklerin zengin bir kaynağı olup, gallik asit, kateşin ve epikateşin gibi yüksek derecede antioksidan üç maddeyi bir arada bulundurmaktadır. Antioksidan potansiyeli en yüksek ilk elli gıda arasında yer alan Urfa fıstığı ağaç yemişleri arasında suda ve yağda çözünen antioksidanlar açısından en zengin ve en çok çeşitliliğe sahip olup antosiyanin içeren tek yenilebilir kuruyemiş olma özelliğine sahiptir. Urfa fıstığı; antikanser potansiyeline sahip isoflavonlar ve transresveratrol gibi önemli bioaktif polifenol bileşikler de içermektedir. Urfa fıstığının dâhil olduğu Pistacia cinsinin bazı türleri halk arasında diş, gastrointestinal, karaciğer, idrar yolu ve solunum yolu rahatsızlıklarının iyileştirilmesinin yanında ayrıca afrodizyak, antiseptik ve antihipertansif etkilerinden dolayı çeşitli amaçlarla kullanılmaktadır. Birçok çalışmada Urfa fıstığının anti-inflamatuar aktivite, glisemik kontrol ve endotel fonksiyonu gibi vücut sağlığının idamesini sağlayan mekanizmaların devam etmesine yardımcı olduğu, ayrıca; aterogenezde LDL kolesterolünün okside olmasını engellediği, kanser ve kardiyovasküler hastalık gibi kronik hastalıklara karşı koruyucu rol oynayabildiği tespit edilmiştir. Diğer fıstık türleri arasında 10,05 mg/gram ile en yüksek potasyum içeriğine sahip olan Urfa fıstığı sodyum içerikli tuz ihtiyacını azaltarak tansiyon üzerinde dengeleyici bir rol oynayabilmektedir. Tohum ve özellikle dış kabuk ekstresinin birçok hastalığa olumlu etkisi olan Urfa fıstığının özellikle dış kabuğunun değerlendirilmesi açısından bu derleme önemlidir.

Aim: To investigate whether erdosteine supplementation following selenite exposure affects oxidant-antioxidant equilibrium and prevents cataract formation in rat pups.Methods: Thirty-nine Wistar-albino rat pups were divided into 3 groups. In Group 1 (n=16) only s.c saline was injected. In Group 2 (n=10) subcutaneous (s.c.) sodium selenite (30 nmol / g body weight) was injected on postpartum day 10. In Group 3 (n=13) s.c. sodium selenite (30 nmol/g body weight) were injected on postpartum day 10 and oral erdosteine (10 mg/kg body weight, daily for one week) was administered by gavage thereafter. The development of cataract was assessed weekly. The density of cataract was graded by slit-lamp biomicroscopy. On day 21, the blood was collected and lenses were removed. Oxidative stress index (OSI) and total antioxidant capacity (TAC) were determined in the lenses of the rat pups. Paraoxonase-1 (PON 1) activity was determined in the sera.Results: All of the lenses of rat pups in Group 1 remained clean. All rat pups developed dense nuclear opacity in Group 2. Eight out of 13 rat pups developed slight nuclear opacity in Group 3. Differences among the groups were statistically significant (p<0.05). Group 2 lenses had higher mean OSI level than that of Group 3 lenses (p=0.003). Group 2 lenses lower mean TAC levels than that of Group 3 (not significant). Mean PON 1 level of Group 2 was lower than that of Group 3 (p<0.05).Conclusion: Erdosteine diminishes the incidence of cataract due to its protection of the antioxidant defense system.