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This work aims to study the influence of gamma irradiation on some bismuth borate glasses doped with different transition metal oxides (CuO, CoO, ZnO and CdO). The structure of the glass matrix was analyzed by Fourier transform infrared and ultraviolet-visible spectroscopy before and after gamma irradiation at doses of 1 kGy, 20 kGy and 50 kGy. Additionally, the attention is made in determining the attenuation parameters against gamma rays. Mass attenuation coefficient determined experimentally and calculated theoretically by WinXCom program, which is a significant program in the field of radiation shielding. It is found that the attenuation parameters of gamma ray have higher values of the sample without transition metal oxides and the other doped with CdO. From hardness measurements before and after irradiation, it was found that hardness increases with the addition of transition metal oxides to glass. The results of different techniques show that these glasses possess a slight effect on the network structure of the samples against gamma irradiation. So, it can be used in different applications of nuclear fields.