Three isolates of Actinomycetes namely MA02, MA03, and MA04 were isolated from an unidentified sponge, Lemukutan Island, Indonesia. They were identified as Streptomyces based on Bergey’s Manual of Determinative Bacteriology. In the preliminary screening, all of the isolates showed antimicrobial activities towards 13 microorganism tests. However, the activities were not similar when their extract produced from various medium and incubation time were tested. Their cultivation was conducted on M13 agar with incubation time for 3 days, M13 broth with incubation time for 4 days, M2+ broth with incubation time for 7 days and M2+ broth with incubation time for 9 days. The result of antimicrobial assay showed difference in the ability to inhibit the microorganism tests. The best antimicrobial activity of the extracts based on cultivated condition (medium and incubation time) for Streptomyces MA02, Streptomyces MA03, and Streptomyces MA04 were M2+ broth for 3 days, M2+ broth for 9 days and M2+ broth for 9 days respectively.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.