Water deficit is considered as a major limiting environmental factor for plant growth and yield. To ameliorate the adverse effects of water restriction, an experiment was conducted in the research field of Mohaghegh Ardabili University in two successive years (2014 and 2015). Foliar spraying of different concentration of epibrassinolide (EBL) (0, 10 −8 , and 10 −7 M) and ascorbic acid (AsA) (0 and 10 mM) was carried out and water-stress trials included 50 and 100 mm evaporation from class A pan. Water stress significantly enhanced essential oil content, but reduced capitula yield and relative water content (RWC) of leaves. Water-stress damage ameliorated by foliar application of 10 mM AsA with 10 −7 M EBL and the essential oil yield and antioxidant enzymes activity improved significantly. Enhancing of malondialdehyde (MDA) content and electrolyte leakage indicates that water-deficit stress caused oxidative damage to the membrane by enhancing hydrogen peroxide (H 2 O 2) level. Combined-application of regulators significantly declined the amounts of H 2 O 2, MDA, and electrolyte leakage under water stress. Antioxidant enzymes activity and also proline and protein content were enhanced by drought stress as well as regulators. Also, the application of EBL and AsA induced tolerance to water deficit and reduced the reactive oxygen species by increasing antioxidant enzymes activity and osmotic adjustment.
Reduction of seedling growth is a consequence of seed deterioration. An experiment was conducted to evaluate the effects of duration of seed aging on soybean seed germination characteristics. Experiment conducted as completely randomized design with 4 replications. The seeds of 'DPX' line of soybean with 15-16% moisture were incubated for 3, 7, 10, 14, 17 day at 40°C in sealed containers for ageing treatment and non aged seeds were considered as control. Results showed that rapid aging treatment has a significant effect on studied parameters. Germination percent (GP), means of daily germination (MDG), means time to germination (MTG), coefficient of velocity of germination (CVG) and germination index (GI) decrease significantly. Also, germination uniformity (GU) was affected by deterioration time. Abnormal seedling (ABS) percentage increased. Results shows that rapid aging experiment could be a useful test for determine soybean seed quality.
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