Two factorial completely randomized design trials 2×2 and 2×2×2 were conducted to evaluate the effect of a blend of essential oils and organic acids (Biacid TM ) in broiler breeder diets at two levels, two dietary non-starch polysaccharides (NSPs) levels and in ovo injection of Biacid TM on their progenies performance, respectively. 240 broiler breeders of Ross 308 strain were fed from the age of week 44 th for 12 weeks in four groups. 120 produced eggs from each group were divided in two groups of 60 eggs for injecting by 0.5 ml of Biacid TM or distilled water. Injection was done during transferring from setter to hatcher in day 18 th of incubation. Twenty-five cockerels from each of 8 treatments were housed into separate pens. Using Biacid TM and high NSPs in broiler breeders' ration affected hatchability, embryo mortality, weight of day old chicks and progenies' carcass yield significantly (p＜0.05) whereas in ovo injection of Biacid TM did not show significant effects in this regards (p≥0.05). Offspring's abdominal fat was neither affected by broiler breeders' rations nor in ovo injection of Biacid TM (p≥0.05). Biacid TM and high NSPs content in broiler breeders' ration affected all primary and secondary humoral immune responses of progenies against sheep red blood cells (p＜0.05). In ovo injection of Biacid TM increased the primary IgG, primary IgT and secondary IgG responses (p＜0.05). The interaction of the effects of Biacid TM and high NSPs in broiler breeders' ration and also in ovo injection of Biacid TM affected progenies' weight gain, feed intake, feed conversion ratio and European production index significantly (p＜0.05). It seems that using Biacid TM in broiler breeders' diet can modify the undesirable effects of high NSPs content of breeders' ration on performance of their offspring.