Testicular torsion is an urgent condition in pediatric urology. Testicular torsion refers to testicular rotation around its vascular axis (spermatic cord), which results in obstruction and stoppage of blood flow and eventually the loss of living tissue (infarction). Ischemic condition causes damage to the testes due to the blood flow disruption. Furthermore, reperfusion of blood flow after ischemia (detorsion) can exacerbate the damage to the testis. Vasopressin as an antidiuretic hormone (ADH), is a pituitary hormone found in most mammals including humans. It is mainly made in supra-optic nuclei. Vasopressin is a polypeptide hormone with nine amino acids and is similar to the oxytocin hormone. In an experimental study, we found that vasopressin possesses intracellular mechanisms similar to the protective preconditioning mechanism which can reduce damages caused by ischemia-reperfusion.