Excess of copper ion (>2 mg/L) in water is toxic to human beings and the ecosystem. Various water treatment technologies for copper remediation have been investigated in the past. Along with industrial effluents, Bordeaux mixture is also a noteworthy copper contamination source in the agricultural ecosystem. In our study, the biosorbent efficiency of dried orange peel was investigated through an environment-friendly process for the removal of cupric ions. Effects of pH, adsorbate concentration, adsorbent dosage, and temperature for the removal of Cu (II) were studied. Slightly acidic environment (pH = 6) was found to be optimum for removal of copper. The equilibrium data were well fitted with the Langmuir and Freundlich isotherms. The surface morphology of the adsorbent was studied using scanning electron microscope. Crystalline nonhomogenous surface was observed after copper adsorption. Desorption study indicated that 0.1N HSO is the best eluent for the removal of adsorbed copper from the powdered dried orange peel.
The dataset presented in this article is associated with the TMT (Tandem mass tag) labeled proteomics of chili pepper plant (
Capsicum frutescens
) infested by a broad mite (
Polyphagotarsonemus latus
). Data was captured using a nano liquid chromatography system coupled with high-resolution Orbitrap FusionTribridmass spectrometer. Proteomics data was analyzed using the Proteome Discoverer version 2.4 tool using MASCOT and SequestHT algorithms. We have identified a total of 5,807 proteins supported by 48,555 unique peptides and 1,279,655 peptide-spectrum matches. Individually, 5,186 proteins were detected in healthy leaf samples, 5,193 in infested leaf sample, 5,194 proteins in healthy meristem sample, and 5,196 proteins in infested meristem samples. Datasets obtained from reciprocal blast against the
Arabidopsis thaliana
proteome database enabled the prediction of protein-protein interactions, and subcellular localization of differentially expressed proteins, which are also included in this article. Data presented in this article has been deposited in the ProteomeXchange Consortium via the PRIDE repository, which can be accessed through the accession ID: PXD018653.
Orchids with their sheer variety of species are amazing, a major sharer in global floriculture trade. Papilionanthe Miss Joaquim, well known hybrid orchid, also recognized as official National flower of Singapore is a resilient, sun loving orchid, which blossoms throughout the year. Though a seed pod derived from a single flower contains millions of dusty seeds, due to lack of endosperm, seeds cannot germinate without the help of symbiont fungus. Commercial production of seedlings thus remains challenging. Therefore, in this study, we report a novel asymbiotic seed germination protocol standardized for ‘Papilionanthe Miss Joaquim’ (a hybrid of Papilionanthe hookeriana x Papilionanthe teres) which could be adapted for mass cultivation in a commercial setup. Seed of Papilionanthe Miss Joaquim were treated with 0.1% H2O2 and 0.1% KNO3 to increase the germination rates. Seed pods were also subjected to various regimes of surface sterilization methods to reduce contaminations. Employing Carbendazim (1% w v-1), Tricyclazole (1% w v-1), Sodium hypochlorite (0.5% v v-1) and Ethanol (80% v v-1) in surface sterilization process, resulted in highest percentage of aseptic cultures. Seeds were inoculated on to Murashige and Skoog (MS) medium supplemented with BAP (6-Benzylaminopurine), NAA (1-Naphthaleneacetic acid) and 2,4-Dichlorophenoxyacetic acid (2,4-D) combination of 3 mg L-1 BAP (6-Benzylaminopurine) + 0.5 mg L-1 NAA (1-Naphthaleneacetic acid) found to be most effective to induce germination (84.67 ± 3.2%). Subsequently, germinated seedlings were subjected to different levels of BAP and NAA levels to achieve the highest number of plantlets. thus, multiplied plantlets were later subcultured onto MS medium containing 0.5 mg L-1 BAP and 3 mg L-1 NAA to induce rooting. Consequently, developed plantlets were acclimatized on a substratum containing coconut husk and charcoal pieces.
Agro-pollution caused by persistent pesticides is an increasing environmental concern. The misuse of pesticides resulting in reduced clearance has prompted an urgent need for developing removal methods. In this regard, biosorption using algae is an attractive option. Algal biomass has been an effective demonstrator of heavy metal bioremediation. Carbendazim is a systemic pesticide used in controlling plant diseases. It has been reported to show nematicide effects making it severely toxic to earthworms. It is a classified Group C Human Carcinogen because it causes chromosomal loss and non-disjunction in mammalian reproductive cells. This investigation estimates the biosorption efficiency of lyophilised chlorophycean Chlorella thermophila (Accession number: MN006612) biomass on carbendazim. Bio-adsorption has shown to increase with pH, showing maximum adsorption at pH 10.
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