The objective of the study was to evaluate the performance of sex sorted semen that was produced for the first time in India at BAIF semen production facility, Pune, using the technological processes of sexing technologies, USA, under tropical small holder dairy production systems. Additionally, a notable point is that the semen was produced from the population of Indian breeds of cattle, its crosses and buffaloes which co-evolved and adopted to the country' agro climatic conditions. 21,012 artificial insemination data and 2,284 calving records were included in the analysis. The parameters evaluated were conception rate and sex ratio. The statistical analysis was performed using the logistic regression method to estimate the effect of factors on conception rate. The effects studied were states, order of lactation, month of insemination, and breed of bull. The overall mean conception rate was 39.92 ± 0.5 per cent, while the sex ratio of female to male was found to be 90.9 %. All of the effects included in the study significantly influenced the conception rate. Odisha state reported higher conception rate while low conception rate was found in Uttarakhand state. Conception rate increased gradually from November to April and declined later. Heifers showed higher conception rate than cows with subsequent lactations, however the difference among the other lactations was very less. The Holstein Friesian crossbred bulls with 75 per cent exotic blood level showed better conception rate than the rest of the breeds. Present study highlights the technical performance and merits of using sex sorted semen in the Indian dairy sector as a way to increase the country' milk production and reduce the burden of maintaining male calves at farm level.