Objectives:The aim is to evaluate the anticancer potential of Annona squamosa (AS) against 7, 12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinoma in rats. Methods:The tumor was induced in Sprague-Dawley rats by gastric intubation of 25 mg DMBA in 1 ml olive oil. After 3 months of induction period, the methanolic extract of AS at different doses of 100, 150, 200, 250, and 300 mg/kg body weight were administered orally a dose per day to mammary carcinoma-bearing rats for 45 days. The serum and tissue levels of glycoprotein components as well as the activities of marker enzymes and lysosomal enzymes were measured in DMBA-induced mammary carcinoma-bearing rats.Results: Administration of AS resulted in decrease in the levels of marker and lysosomal enzymes and also alterations in the body weight and tumor volume were also restored to near normalcy in a dose-dependent manner. The results of the present study indicate that AS has anticancer effect and it exhibits its potential effect at the dosage of 200 mg/kg body weight in mammary carcinoma-bearing rats. Conclusions:Based on the results obtained, it can be concluded that the methanol extract of AS possesses anticancer properties. Further study is needed to isolate the active principle of this extract responsible for anticancer activity to develop the future pharmaceuticals.

Objective: To study the restorative effect of Tridham (TD) and 1,2,3,4,6-penta-o-galloyl-β-D-glucose (PGG) on 7,12-dimethyl benz(a)anthracene(DMBA)-induced mammary carcinoma in female Sprague-Dawley rats.Methods: Rats were divided into seven groups of six animals each. Group I rats served as control. Group II - mammary carcinoma was inducedby DMBA. Group III and Group IV were induced with DMBA and subsequently treated with TD and PGG, respectively, for 48 days. Group V wastreated with DMBA and subsequently with a standard drug, cyclophosphamide (CYC). Group VI and Group VII were given TD and PGG alone,respectively, for 48 days. After the experimental period, the levels of lipid peroxides (LPO), activities of enzymic and non-enzymic antioxidantssuch as superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, reduced glutathione, vitamin C, and vitamin E wereevaluated in the breast tissue of control and experimental rats. Levels of LPO, marker enzymes such as 5’-nucleotidase and lactate dehydrogenase,were also evaluated.Results: The levels of enzymic and non-enzymic antioxidants were decreased in DMBA-induced rats when compared to control rats. The levels oftumor markers were increased in DMBA-induced rats when compared to control rats. These parameters were restored to near normal levels ontreatment with TD and PGG.Conclusions: The results suggest that TD and PGG have a cytoprotective role in DMBA-induced breast cancer-bearing rats. The effect of TD and PGGwas found to be more pronounced than CYC, a standard drug.Keywords: Breast cancer, Tridham, Penta galloyl glucose, Antioxidants, Tumor markers, Sprague-Dawley rats.