Vibrio vulnificus is a Gram-negative, opportunistic human pathogen capable of causing life-threatening septicaemia, wound infections, and gastroenteritis, especially in immunocompromised individuals. Two cases of V. vulnificus-associated wound infection occurring in diabetic patients are reported here. The pathogen was detected by PCR targeting species-specific marker gyrB and virulence markers, including repeats in toxin (rtxA) and hemolysin (vvhA), but the causative agent could not be cultured. Genotyping based on the virulence-correlated gene revealed that the V. vulnificus detected in this study belonged to the vcg-C type, which is commonly associated with clinical cases. This report highlights the clinical applicability of PCR-based methods in the detection of V. vulnificus in culture-negative cases. Such methods may add a very useful clinical dimension to currently used diagnostic practices.
Despite improvements in access to health care in India, inequalities related to socioeconomic status, geography and gender continue to exist. This is further compounded ABSTRACT Background: Remedial action that a person takes to treat his or her perceived illnesses, is called health-seeking behaviour. Understanding health seeking behaviour (HSB) in a community and the factors which influence their decision, are crucial for planning a "need based" comprehensive health care programmes for the people, especially for underprovided and rural population. The objective of present study was to determine HSB and its determinants in a defined rural population. Methods: A community based cross -sectional study was conducted in rural field practice area of A. J. Institute of Medical Sciences and Research Centre, Mangalore (Karnataka) from 1st October 2017 to 31st July 2018. A total of 866 heads of households were included in the study, using universal sampling method. A pre-tested, validated questionnaire was used to collect the data. p values of <0.05 were considered significant. Results: Among 866 participants, almost all (98.03%) heads of families were males, while majority of them (35.68 %) were farmers. Further, majority (48.15%) of the households preferred to visit government hospitals for their ailments, while among those visiting private practitioners, majority of them (55.54%) preferred Allopath system of medicine. Health insurance subscription rates were found to be low (18.93%). Main determinants of HSB were observed to be cost of treatment, convenience of approach, quality of services and life threatening emergencies. Conclusions: Present study brings out higher utilization of government health care facilities among the study subjects, while cost of treatment and life threatening emergencies were the main predictors of HSB.
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