The prospection of bioherbicides has been an alternative to weed control, aiming at mitigating chemical risks to human, animal and environmental health due to extreme use of synthetic herbicides. In the present study, various fungi were isolated from plants with symptoms of fungal diseases for bioherbicide purposes against weeds ( Urochloa plantaginea , Euphorbia heterophylla and Bidens pilosa ). Fungi isolated were identified by molecular methods and enzymatic products obtained by fungi fermentation (cellulase, lipase, peroxidase, and amylase) were quantified. Bioherbicide selectivity study was performed on crops (soybean and corn), as well as on resistant weeds. Among the isolated fungi, Fusarium oxysporum , Fusarium ploriferatum, and Trichoderma koningiopsis presented bioherbicide potential. T. koningiopsis , in particular, presented the highest effect on Euphorbia heterophylla (popular name - Mexican fire plant), causing up to 60% of foliar damage, without presenting phytotoxicity against corn crop. New perspectives for weeds control and their use in corn crops were prospected, considering the bioherbicide selectivity described in this study.

As plantas daninhas Digitaria ciliaris (milhã) e Ipomoea indivisa (corda-de-viola) destacam-se por ocasionarem elevados prejuízos à cultura do milho. Objetivou-se com o trabalho avaliar a interação competitiva relativa entre híbridos de milho na presença de milhã e corda-de-viola, por meio de experimentos em série substitutiva. Os experimentos foram instalados em delineamento experimental de blocos casualizados, com quatro repetições. Primeiramente, tanto para os híbridos de milho (Agroeste - AS 1551 PRO 2, Morgan - MG 300 PW, Nidera - NS 92 PRO and Syngenta - Velox TL) quanto para as plantas daninhas (milhã e corda-de-viola) determinou-se a densidade de plantas em que a produção final se tornou constante (20 plantas vaso-1 ou 463 plantas m-2). Posteriormente foram instalados oito experimentos para avaliar a habilidade competitiva dos híbridos de milho com as plantas daninhas, ambos conduzidos em série de substituição, nas diferentes combinações das espécies, variando-se as proporções relativas (100:0, 75:25, 50:50, 25:75 e 0:100%) de plantas vaso-1 (20:0; 15:5; 10:10; 5:15 e 0:20). A análise da competitividade das espécies foi efetuada por meio de diagramas aplicados a experimentos substitutivos e também pelos índices de competitividade relativa. Aos 50 dias após a emergência das espécies efetuou-se a aferição da área foliar e da massa seca da parte aérea das plantas. Os híbridos de milho e as plantas daninhas foram afetados negativamente, sendo a competição interespecífica a mais prejudicial para as espécies. A cultura apresentou os maiores índices de competitividade, de crescimento, de dominância e de agressividade. Ao se comparar as espécies entre si, o milho foi mais competitivo do que as plantas daninhas.

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As plantas daninhas ocasionam efeitos negativos na produtividade e na qualidade dos grãos da cultura do trigo quando não controladas de modo eficiente. Assim sendo, objetivou-se com os trabalhos avaliar a seletividade e a eficácia de herbicidas aplicados para o manejo de plantas daninhas infestante da cultivar de trigo TBIO Sinuelo. Foram instalados dois experimentos, um para avaliar a seletividade e outro a eficácia de herbicidas aplicados em trigo, em blocos casualizados, com quatro repetições. Os tratamentos testados foram: iodosulfuron, pyroxsulam, metsulfuron-methyl, 2,4-D, clodinafope-propargil, [bentazon + imazamox], saflufenacil, bentazon, carfentrazone-ethyl, imazamox, pendimethalin, [sulfentrazone + diuron], mais duas testemunhas uma capinada e outra infestada. Os herbicidas que ocasionaram as maiores injúrias ao trigo foram o imazamox e a mistura comercial de [bentazon + imazamox] com fitotoxicidade próxima a 50%. A aplicação da mistura comercial de [sulfentrazone + diuron] demonstrou a maior produtividade de grãos do trigo. O uso de clodinafope-propargil e pyroxsulam apresentaram os melhores controles de Lolium multiflorum, com 100 e 98,25%, aos 21 dias após a aplicação dos tratamentos, respectivamente. Ocorreu controle acima de 90% de Raphanus raphanistrum com uso de iodosulfuron, pyroxsulam, metsulfuron-methyl, 2,4-D, [bentazon + imazamox], saflufenacil, bentazon e carfentrazone. O clodinafope-propargil demonstra o maior controle para o azevém e o iodosulfuron-ethyl, pyroxsulam, metsulfurom-metílico, 2,4-D, saflufenacil e carfentrazone para o nabo. O pyroxsulam foi o tratamento que demonstrou, nos dois experimentos, os melhores resultados para os componentes da produção, especialmente para a produtividade da cultura.

The arrow-leaf sida (Sida rhombifolia), in addition to competing with soybeans, which causes productivity losses, is also host to the whitefly, which transmits viruses. The aim was to evaluate the competitive ability of soybean cultivars in the presence of an arrow-leaf sida biotype, with different proportions of plants in the association. The experiments were conducted in a completely randomized design, with four replications. Tested competitors included arrow-leaf sida with the four soybean cultivars in proportions crop:weed of 100:0; 75:25; 50:50; 25:75; and 0:100. Competitiveness analysis was carried out using diagrams applied to replacement series experiments in addition to the relative competitiveness indices. At 50 days after the emergence of the species, the variables height, diameter, leaf area, and dry mass of shoots were evaluated. Competition occurred between soybean cultivars in the presence of arrow-leaf sida, regardless of the plant proportion, causing reductions in all variables evaluated. Interspecific competition causes greater damage to al variables studied than intraspecific competition. There was competition for the same resources between soybean and arrow-leaf sida.

The intensive and abundant use of synthetic herbicides has been questioned in recent decades due to the strong dependence and also the resistance effects that are identified in weeds. Several grain crops suffer from the weed control system because many of the weeds are already resistant to the main herbicides that are used. In recent years, there has been a large gap in the market without the addition of new synthetic herbicides with mechanisms of action that differ from those already existing. The objective of this short piece is to address and overcome this challenge and bring an innovative and alternative solution that proposes a synergistic action system between bioherbicides produced by the fungus Trichoderma koningiopsis and synthetic herbicides (2,4-dichlorophenoxyacetic acid, glyphosate, and ammonium glufosinate). The plants included in this study were Bidens pilosa (amor seco, or in the United States, beggar ticks or Spanish needle), Euphorbia heterophylla (adeus-brasil), and Conyza bonariensis (margaridinha-do-campo, or, in the United States, hairy fleabane or asthmaweed). It was verified that, in the application of the biocomposites in the presence of chemical herbicides, potentiation of the phytotoxic action (100%) occurred under the target plants, emphasizing phytotoxicity to the weed, C. bonariensis, which is currently resistant to available herbicides. The bioherbicides studied have promising characteristics to be explored in the biocontrol of weeds.

The present study was designed to evaluate the scaling process used to obtain fungi and enzymes from Trichoderma koningiopsis to produce bioherbicides with low levels of genotoxicity using a stirred-tank bioreactor. We measured fungal biomass and activities of enzymes (amylase, cellulase, lipase and peroxidase). Fermentative products were applied to weeds (Bidens pilosa and Euphorbia heterophylla), and the bioherbicide effect was evaluated. We found greater elevations of peroxidase and lipase activity in the scaled-up models than in those at small scale (0.15 L), even with less biomass, suggesting that the scaling was cost-beneficial in terms of enzymatic yield. Bioherbicide activity tests showed up to 100-80% phytotoxic effects on weeds that were resistant to synthetic herbicide. A genotoxicity study of the fermented extract performed in Allium cepa meristem cells showed low genotoxicity and no DNA damage. Our findings point to new perspectives regarding bioherbicides derived from fungal fermentation and enzyme yield technology, aimed at sustainable and ecofriendly agriculture.