AimTo investigate whether the emulsification of conventional silicone oils can be reduced by adding small amounts of silicone molecules of a very long chain length.MethodsSiluron 1000, Siluron 2000, Siluron 5000, Acri.Sil-Ol 5000, Oxane 5700, Densiron 68 LV, Densiron 68 and Densiron 68 HV (0.5 ml) were each tested along with either plasma or serum (0.5 ml) in a glass cuvette. Emulsification was induced by sonication and documented by photography. The total area of emulsified oil was assessed using the ImageJ software.ResultsThe addition of small amounts of very-long-chain silicone molecules significantly reduced the emulsification for 1000 cSt silicone oil (Siluron 2000) and for 1000 cSt silicone oil with an admixture of F6H8 (Densiron 68 HV).ConclusionNew low-viscosity silicone oils show a reduced emulsification similar to that of 5000 cSt oils. In future, it may be possible to avoid using 5000 cSt oils. The findings may foster silicone oil surgery in general, and in particular the application of small-incision techniques.
Aus Protoplasten von Candidu lipolytiea, Stamm 7 -3, wurden nach osmotisohem Schock dnrch Differentialzentrifugation bei 1500, 8000 und 16 000 g subzellulare Partikeln gewonnen. D i e Fraktionen katalysierten mit NADH als Cosubstrat den enzymatischen Abbau von n-Hexadecan-l-14C. Bei Substratkonzentrationen von 0,46 bis 1,05 pmol/ml betrugen der Abbau 20 bis 75,8% und die Umsatzrate 3,5 bis 17,9 ymolmg Pr0tein-l-h1. Die Reaktion war bereits nach 10 bis 15 min beendet. Die lipidloslichen Oxydationsprodukte wurden siinlenchrornatographisch vom Restalkan abgetrennt. In der wiilrigen Phase des Inkubationsansatzes konnten hydrophile Reektionsprodukta naohgewiesen werden. AuBerdem d e n die von den subzelluliiren Pastikeln absorbierten radioaktiv markierten Substanzen gemessen.
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