Obtaining edible oil from animals and plant sources is an ancient practice of the people in the tropical regions of the globe. Industrialization of oil and fats began with the creation of a cotton seed mill, due to higher demand several different plants have been studied to get good and healthy oil and fats. In this research the fixed oil from the berries of Sea Buckthorn was characterized and studied for its fatty acid composition by biochemical tests and Gas Chromatography Mass Spectroscopy (GCMS). The chemical characteristics of oil from seeds showed the saponification value 248.36, iodine value 80.49 and free fatty acid value 11.28%. While the identified fatty acids were palmitic acid (C16, 0) 24.98%, oleic acid (C18, 1) 20.97%, linoleic acid (C18:2) 11.51%, linolenic acid 4.31(C18:3) %, and stearic acid (C18, 0) 0.29%. The fatty acid profile and physico chemical value of the oil showed that oil has health promoting chemistry and good source of energy. These findings provide essential data explaining that the oil obtained from the berries is nothing less than any other good quality of edible oil.
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