Aim: to assess nurses' knowledge and practice for patients undergoing bronchoscopy procedures and design suggested nursing care guidelines for those nurses. Design: descriptive research design was utilized in this study. Setting: The study was conducted on 40 nurses at bronchoscopy unit of the chest department, at Assiut University hospital. Tools: A structured interview questionnaire sheet for nurses. Including: Demographic data, an observation checklist for nurses and develop suggested nursing care guidelines. Results: Fifty two of studied nurses had poor knowledge score, and regarding the total score of nurses' practice, (87.5%) of them was satisfactory. In addition, there was no correlation between nurses' knowledge and practice total scores and their demographic data except between good knowledge level score and their ages and marital status. Conclusion: the studied nurse practice levelwas satisfactory while their knowledge scores were not in the same line.