Affectivity ofPreliminary bioassays were conducted to determine the virus activity against the 1 st and 2 nd instar larvae of the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). First instar larvae were more susceptible to the virus than the second instar larvae. Mortality response of the larvae was greater at the highest concentration of the virus than at lowest concentrations. The lethal concentration that killed 95% of the tested larvae (LC95%) was 2.32 x 10 , 2 x 10 4 , 2 x 10 5 and 2 x 10 6 PIB/ml). However, second instar (2 nd ) larvae were less susceptible to the virus than the first instar larvae with the mortality rate of 0, 4, 9.39, 25.33, 43.24, and 71.63%, for the same tested concentrations, respectively. In control treatment, no mortality was recorded. The high concentration levels caused a high rate of mortality. The same trend of mortality is similar to those found by other studies.
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