Aims/hypothesis The study aimed to evaluate the efficacy of recombinant adenovirus expressing αA-crystallin (Ad-αAc-Gfp) in reducing pericyte loss within retinal vasculature in early diabetes. Methods Diabetes was induced by streptozotocin injection into C57BL/6 mice. Ad-αAc-Gfp was delivered by intravitreous injection to the right eyes of mice 2 weeks before induction of diabetes. Vascular leakage was determined by fluorescent angiography, Evans Blue leakage assay and leucocyte adhesion test. Production of αA-crystallin was analysed by immunoblotting and double immunostaining and pericyte loss was analysed by pericyte count. Results Vessel leakage and pericyte loss were observed in the streptozotocin-induced diabetic retina. Decreased abundance of αA-crystallin in retinas 2 and 6 months after the induction of diabetes was confirmed by two-dimensional electrophoretic analysis, immunoblotting and RT-PCR. Double immunofluorescence staining for αA-crystallin and NG2 chondroitin sulphate proteoglycan revealed that αA-crystallin was predominantly produced in the retinal pericyte and that the number of αA-crystallin-producing pericytes decreased in the diabetic retina. Retinal infection with Ad-αAc-Gfp led to decreased pericyte loss and vascular leakage compared with control. Conclusions/interpretation Intravitreal delivery of Ad-αAc-Gfp protects against vascular leakage in the streptozotocininduced model of diabetes. This effect is associated with the inhibition of diabetic retinal pericyte loss in early diabetes, suggesting that αA-crystallin has a role in preventing the pathogenesis of early diabetic retinopathy.
KR. Increased expression and activity of 12-lipoxygenase in oxygen-induced ischemic retinopathy and proliferative diabetic retinopathy: implications in retinal neovascularization. Diabetes 2011;60:614-624 Several editorial errors occurred in the article listed above. On page 615, the sentences "Additional experiments were performed using db/db mice retinas" and "The streptozotocin-induced db/db mice were maintained for 8 weeks, then one eye of each animal was processed for frozen sections and the retina of the other eye was frozen for protein analysis" should read, respectively, as follows: "Additional experiments were performed using diabetic mice retinas" and "The streptozotocin-induced diabetic mice were maintained for 8 weeks, then one eye of each animal was processed for frozen sections and the retina of the other eye was frozen for protein analysis." On page 619, the sentence "It recently was reported that retinas from nondiabetic or db/db mice neither produce leukotrieness nor 5-lipoxygenase mRNA (37)" should read as follows: "It recently was reported that retinas from nondiabetic or diabetic mice neither produce leukotrienes nor 5-lipoxygenase mRNA (37)." The online version reflects these changes.
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